中国畜牧兽医2011，Vol.38Issue(7)：193-195,3.
驽巴贝斯虫病PCR检测方法的建立和应用
Application and Development of PCR Assay to Detect Babesia caballi
摘要
Abstract
The 18S rRNA gene recently discovered was shown to be species-specific. A pair of primers was designed to specifically amplified a 452 bp fragment. The PCR result of specificity assay showed that one references B. Caballi could be detected by the PCR test,but no amplification was observed when other five bacterial species isolated from T. Equi,B. Bigimina,B. Motasi, B. Ovata, B. Major tissue were detected. And the sensitivity result showed that the minimum dose of B. Caballi that could be detected by PCR assay was 0. 01 fg/μL,45 clinical sample, from horses farm in China, doubtedly infected with B. Caballi were tested by PCR. The results showed that 12 positive samples could be detected by the PCR assay. At the same time,microscope were also used for the clinical samples. The test revealed that the sensitivity of the PCR assay was higher than that the microscope test.关键词
驽巴贝斯虫/PCR诊断方法/应用Key words
Babesia caballi /PCR diagnosis method /application分类
农业科技引用本文复制引用
罗金,刘光远,谢俊仁,田占成,沈辉,王芳芳,孙晓林,陈荣贵,王海军..驽巴贝斯虫病PCR检测方法的建立和应用[J].中国畜牧兽医,2011,38(7):193-195,3.基金项目
甘肃省科技重大专项(092NKDA031). （092NKDA031）
