中国药房2011，Vol.22Issue(39)：3704-3706,3.
微乳液相色谱法同时测定大黄中5种蒽醌类衍生物的含量
Content Determination of 5 Anthraquinone Derivatives in Rhei Radix Et Rhizoma by Microemuision Liquid Chromatography
摘要
Abstract
OBJECTIVE: To develop the method for the content determination of aloe-emodim, rhein, emodin, chrysophanol and physcione. METHODS: Microemulsion liquid chromatography (MELC) was adopted, and mobile phase was microemulsion that was optimized by inspecting the influence factors of separation and retention time. The optimized microemulsion was 2.5% {Wl V) sodium dodecyl sulfate-0.1% (F/F)n-octane-8.0% (VIV) n-butanol-0.5% (V/V) triethylamine (pH adjusted to 3.0 by phosphoric acid). Hypersil ODS2 (250 mm×4.6 mm, 5 μm) column was selected with column temperature of 28 °C at a flow rate of 1 rnL· min-1, and the detection wavelength was set at 254 nm.RESULTS:The linear ranges were 4.7~47.0 μg·mL1 for aloe-emodim (r= 0.999 9), 5.1～51.0 μg·mL-1 for rhein(r=0.999 6), 5.5~55.0 μg·mL-1 for emodin (r=0.999 6), 6.3～63.0 μg·mL-1 for chrysophanol (r=0.999 9), 0.4～40.0 μg · mL-1 for physcione (r=0.998 7). The average recoveries were 98.67% , 97.53% , 101.37% , 99.34% and 99.52%, respectively. RSDs were 0.31%, 0.38%, 0.49%, 0.53% and 0.87% (n=6). CONCLUSION: The method is simple and accurate, and can be used for the content determination of 5 anthraquinone derivatives in Rhei Radix Et Rhizoma.关键词
微乳液相色谱法/大黄/芦荟大黄素/大黄酸/大黄素/大黄酚/大黄素甲醚/含量测定Key words
Microemulsion liquid chromatography/ Rhei Radix Et Rhizoma/ Aloe-emodim/ Rhein/ Emodin/ Chrysophanol/ Physcione/ Content determination分类
医药卫生引用本文复制引用
何素珍,张忠义,张守尧..微乳液相色谱法同时测定大黄中5种蒽醌类衍生物的含量[J].中国药房,2011,22(39):3704-3706,3.基金项目
广东省医院药学研究基金立项课题(2008A002) （2008A002）
