中国药房2011，Vol.22Issue(39)：3709-3710,2.
HPLC法同时测定茵苓祛湿汤中绿原酸、栀子苷的含量
Content Determination of Chlorogenic Acid and Jasminoidin in Yinling Qushi Decoction by HPLC
唐年忠 1吴生齐 1杨立伟2
作者信息
- 1. 广东省食品药品监督管理局审评认证中心,广州市510080
- 2. 广东省药品检验所,广州市510180
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of chlorogenic acid and jasminoidin in Yinling qushi decoction. METHODS: HPLC method was adopted. A Kromasil C18 column was adopted (250 mm×4.6 mm,5 μm)with mobile phase consisted of acetonitrile-0.4% phosphoric acid(13:87)at the flow rate of 1.0 mL·min-1. The detection wavelengths were set at 327 nm for chlorogenic acid and 238 nm for jasminoidin. RESULTS: The liner range of chlorogenic acid was 0.154 4~ 1.235 2 μg(r=0.999 7) with an average recovery of 99.9% (RSD=0.54% ,n=6). The linear range of jasminoidin was 0.363 2~ 2.905 6 ug(r=0.999 9) with an average recovery of 100.0% (RSD=0.51 % ,n=6). CONCLUSION: The method is simple, rapid and accurate for the content determination of chlorogenic acid and jasminoidin in Yinling qushi decoction.关键词
茵苓祛湿汤/绿原酸/栀子苷/含量测定/高效液相色谱法Key words
Yinling qushi decoction/ Chlorogenic acid/ Jasminoidin/ Content determination/ HPLC分类
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唐年忠,吴生齐,杨立伟..HPLC法同时测定茵苓祛湿汤中绿原酸、栀子苷的含量[J].中国药房,2011,22(39):3709-3710,2.
