中德临床肿瘤学杂志（英文版）2011，Vol.10Issue(12)：730-733,4.DOI:10.1007/s10330-011-0876-6
靶向人Pokemon基因的shRNA重组质粒的构建
Construction the hairpin RNA recombinant plasmids targeting human Pokemon gene
Wenmiao Cao 1Tao Zhang 2Buhai Wang 2Xizhi Zhang 1Jinghua Ren 1Jie Yao2
作者信息
- 1. Department of Oncology, Northern Jiangsu People's Hospital, Clinical Medical School, Yangzhou University, Yangzhou 225001, China
- 2. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430023, China
- 折叠
摘要
Abstract
Objective:The aim of this study was to establish the foundation for studying the role of pokemon gene in tumorigenesis and development by constructing recombinant plasmids that can express small interfering RNA (siRNA) targeting human Pokemon gene. Methods: Hairpin siRNA templates targeting Pokemon gene were synthesized and cloned into plasmid vector psiRNA-H1neo. Three vectors derived siRNAs (psiRNA1, 2, 3) and one mocking psiRNAc (as control) were constructed.The recombinant Pokemon siRNA plasmids were constructed and identified using restrictive enzyme analysis and DNA sequencing. Results: Restrictive enzyme analysis and DNA sequencing revealed the successful construction of siRNA expression plasmids. Conclusion: Constructing siRNA templates targeting Pokemon gene may provide us with practical tools for further study the role of Pokemon gene in the development of some diseases and gene therapy of tumor.关键词
Pokemon/ RNA interference/ shRNAKey words
Pokemon/ RNA interference/ shRNA引用本文复制引用
Wenmiao Cao,Tao Zhang,Buhai Wang,Xizhi Zhang,Jinghua Ren,Jie Yao..靶向人Pokemon基因的shRNA重组质粒的构建[J].中德临床肿瘤学杂志（英文版）,2011,10(12):730-733,4.
