北京大学学报（医学版）Issue(5)：730-734,5.DOI:10.3969/j.issn.1671-167X.2011.05.019
胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白骨修复材料的性质评估
Properties evaluation of collagen-hydroxyapatite-chondroitin sulfate-bone morphogenetic protein bone substitute material
王浩 1张里程 1石涛 1熊琦 1唐佩福1
作者信息
- 1. 中国人民解放军总医院骨科,北京100853
- 折叠
摘要
Abstract
Objective:To construct a new 3D porous bone substitute material with collagen, hydroxy-apatite and chondroitin sulfate, which has the main components of nature bone and the cell growth factor BMP-2 with bone inductive ability. Methods: Collagen-hydroxyapatite-chondroitin sulfate scaffolds were prepared by chemical cross linking and freeze-drying, and bone morphogenetic protein ( BMP) was incorporated into the scaffolds by adsorption. The bone substitute material was investigated by HE analysis, scanning electron microscope ( SEM ) , electron spectroscopy for chemical analysis ( ESCA ) , and X-ray diffraction ( XRD). Rat mesenchymal stem cells ( MSCs) were seeded into the scaffolds and cultured to form cell/scaffold ( CS) constructs in vitro. The ectopic osteoinduction of the scaffolds were evaluated in vivo. Results: The bone substitute material had a porous 3D structure facilitating cells growing into it. Implanted into the muscle, the scaffolds were degraded with the forming of new bone. Conclusion: Our Findings indicate that the bone substitute material has good biocompatibility and its attachment to CS could improve the adhesion and differentiation of cells.关键词
骨代用品/胶原/羟基磷灰石/软骨素硫酸盐类/骨形态发生蛋白质类Key words
Bone substitutes/Collagen/Hydroxyapatite/Chondroitin sulfates/Bone morphogenetic proteins分类
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王浩,张里程,石涛,熊琦,唐佩福..胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白骨修复材料的性质评估[J].北京大学学报（医学版）,2011,(5):730-734,5.
