HPLC法测定知柏地黄丸中菝葜皂苷元的含量OACSCDCSTPCD
Content Determination of Sarsasapogenin in Zhibai Dihuang Pills by HPLC
目的:建立测定知柏地黄丸中菝葜皂苷元含量的方法.方法:采用高效液相色谱法.色谱柱为AglientC18柱,流动相为甲醇-水(95∶5),检测器为蒸发光散射检测器,流速为1.0 mL·min-,进样量为20 μL,柱温为25℃.结果:菝葜皂苷元进样量在0.44～4.35 μg范围内与峰面积积分值呈良好的线性关系(r=0.999 7)；平均回收率为98.63%、98.93%、98.93%,RSD= 1.30%.结论:本法简便快捷,结果准确,重复性好,…查看全部>>
OBJECTIVE: To establish the method for the content determination of sarsasapogenin in Zhibai dihuang pills. METHODS: HPLC method was adopted. The chromatographic separation was performed on Aglient C18 column with mobile phase consisted of methanol-water (95 = 5) using evaporative light-scattering detector (ELSD) at flow rate of 1.0 m·L min-1. The injection volume was 20uL and column temperature was 25℃. RESULTS: The linear range for sarsasapogenin was…查看全部>>
陈骁勇
江苏盐城市第一人民医院,盐城市224001
医药卫生
知柏地黄丸菝葜皂苷元高效液相色谱法含量测定
Zhibai dihuang pill Sarsasapogenin HPLC Content determination
《中国药房》 2011 (40)
3826-3827,2
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