北京师范大学学报（自然科学版）2011，Vol.47Issue(3)：277-280,4.
表没食子儿茶素没食子酸酯(EGCG)与人血清白蛋白相互作用的研究
INTERACTION OF EPIGALLOCATECHIN GALLATE WITH HUMAN SERUM ALBUMIN
摘要
Abstract
The interactions of EGCG (epigallocatechin gailate) with HAS(human serum albumin) were siudied by fluorescence and ultra-violet spectroscopic and Autodock3 procedure. It was found that EGCG quenched the fluorescence intensity of HAS by a sfatic quenching process, which indicated that EGCG hound to one binding site in HAS. Resulting in a ground state complex. The molecular quenching constants (Kq) were 7. 153×105 and 7.886 × 105 L·mol-1. And the binding constants (KA) were 9.511 × 105 and 9. 586 × 105 L· mol-1 at 17 and 37 ℃, respectively. Thermodynamic parameters calculated from van't Hoff equation suggest that the interaction was spontaneous and mainly by an initial hydrophobic association. Based on the theory of Forster nonradiative energy transfer, the distance between the ligand (HAS) and recepior (EGCG) and efficiency of energy transfer were calculated to be 3.02 nm and 0.188, res per lively. Dala obtained by spectroscopic analysis were supported by data from Autodock study.关键词
表没食子儿茶素没食子酸酯/人血清白蛋白/荧光/紫外/分子对接Key words
EGCG/ HAS/ fluorescence/ UV/ autodock引用本文复制引用
朱美霖,陆洁,韩梅..表没食子儿茶素没食子酸酯(EGCG)与人血清白蛋白相互作用的研究[J].北京师范大学学报（自然科学版）,2011,47(3):277-280,4.基金项目
国家科技支撑计划基金资助项目(2008BAI49B04) （2008BAI49B04）
