动物营养学报2011，Vol.23Issue(8)：1273-1279,7.DOI:10.3969/j.issn.1006-267x.2011.08.004
日循环高温对肉鸡组织锌离子浓度及金属硫蛋白含量的影响
Effect of Cyclic High Temperatures on the Zn2+ Concentration and Metallothionein Content in Tissues of Broilers
摘要
Abstract
The experiment was conducted to investigate the effect of cyclic high temperatures on the Zn2+ concentration and metallothionein content in pancreas, liver and duodenum of broiler chickens. One hundred and twenty 32-day-old Arbor Acres male broilers were randomly allotted to 3 groups (normal-temperature group, paired-feeding group and high-temperature group) with 4 replicates in each group and 10 birds in each replicate. The broilers in the normal-temperature group and paired-feeding group were maintained in a controlled environment temperature at 23 ℃, and the paired-feeding group were matched the diet intake of broilers in high-temperature group at the previous day of the experiment. The broilers in the high-temperature group were housed in a controlled environment temperature with daily cyclic high temperature (23℃ - 33℃ - 23 ℃) , and drinked ad libitum. The results showed as follows: after 4 and 8 d cyclic high temperatures, the Zn2+ concentration in plasma, liver, pancreas and duodenum of broilers was significantly decreased (P <0. 05) , and the mRNA expression level of metallothionein gene in pancreas was significantly increased (P <0.05). After 4 d cyclic high temperatures, the metallothionein content and its mRNA expression level in liver and duodenum of broilers were significantly decreased (P <0. 05) , after 8 d cyclic high temperatures, they became the nor-mol levels, but the metallothionein content in pancreas was significantly decreased (P <0. 05); there was no significant differences in the Zn2 + concentration and metallothionein content, and the mRNA expression level of metallothionein gene between the normal-temperature group and paired-feeding group (P >0. 05). In conclusion, cyclic high temperatures significantly decreases the Zn2+ concentration and metallothionein content in liver , pancreas and duodenum of broilers, which is independent of the decreasing of food intake induced by cyclic high temperatures, and the changing trend of metallothionein content is similar with that of zinc concentration in liver, pancreas and duodenum of broilers. [Chinese Journal of Animal Nutrition, 2011, 23(8):1273-1279]关键词
高温/肉鸡/锌离子浓度/金属硫蛋白Key words
cyclic high temperatures/ broiler/ Zn2+ / metallothionein分类
农业科技引用本文复制引用
吴睿,张敏红,冯京海,郑姗姗..日循环高温对肉鸡组织锌离子浓度及金属硫蛋白含量的影响[J].动物营养学报,2011,23(8):1273-1279,7.基金项目
动物营养学国家重点实验室项目(2004DA125184G0807) （2004DA125184G0807）
