医药导报2011，Vol.30Issue(12)：1559-1561,3.DOI:10.3870/yyb.2011.12.006
姜黄素对HaCaT细胞增殖和凋亡的影响
Effects of Curcumin on Proliferation and Apoptosis of HaCaT Cells
王莉 1曹毅 1杨晓红1
作者信息
- 1. 浙江中医药大学第一附属医院皮肤科,杭州,310006
- 折叠
摘要
Abstract
Objective To investigate the effects and possible mechanism of curcumin on proliferation and apoptosis of HaCaT cells. Methods The inverted microscope was used to observe morphology of HaCaT cells co-cultured with different concentrations of curcumin; the MTT assay was taken to check cytotoxic effect of curcumin on HaCaT cells; the flow-cytometric analysis was carried out to test extent of apoptosis; Western Blot was applied to examine Bcl-2 and Bax protein expression. Results The morphology of cells treated with curcumin changed in a dose-dependent manner and part of cells fell off from the vial. No significant difference in MTT assay was showed between group of curcumin at 0. 1 |xmol ? L"1 and the control ( P> 0.05 ),but it was shown for the group of curcumin over 0. 1 |xmol ? L'1 ; Flow cytometry results showed that the apoptosis of HaCaT cells was dose-dependently raised by curcumin;the expression of Bax was elevated and Bcl-2 was dropped. Conclusion Curcumin inhibits HaCaT cells proliferation and elicits their apoptosis, the mechanism of which may be related to the regulation of Bcl-2 and Bax expression.关键词
姜黄素/HaCaT/细胞凋亡Key words
Curcumin/HaCaT/ Apoptosis分类
医药卫生引用本文复制引用
王莉,曹毅,杨晓红..姜黄素对HaCaT细胞增殖和凋亡的影响[J].医药导报,2011,30(12):1559-1561,3.
