武警医学2011，Vol.22Issue(8)：688-692,5.
血清高迁移率族蛋白B1与脓毒症小鼠心肌细胞凋亡的相关性研究
Correlation between HMGB1 and myocyte apoptosis in septic mice
摘要
Abstract
Objective To investigate the correlation between serum HMGB1, the myocyte apoptosis rate and serum BNP in septic mice and to probe the effect of sodium butyrate (SB) on myocyte apoptosis and cardiac function. Methods The septic mouse model was developed by cecal ligation puncture (CLP). 120 mice were randomly divided into three groups of 40 each; sham group, CLP group, and SB group. The serum concentration of HMGB1 and BNP was detected by ELISA at 0,12,24,48,72h separately in each group, and the apoptosis rate of myocytes was measured by flow cytometry at the same time. Both the correlation between HMGB1 and the myocyte apoptosis rate and the correlation between HMGB1 and BNP were studied. Meanwhile, the effect of sodium butyrate treatment on the myocyte apoptosis rate , the serum concentration of BNP and the survival prognosis at 120h was observed. Results The serum concentration of HMGB1 began to elevate after CLP 12h. Positive correlation was found not only between HMGB1 and the myocyte apoptosis rate, but also between HMGB1 and BNP at 12,24,48,72h (P<0. 05). Sodium butyrate decreased the apoptosis rate of myocytes and the concentration of BNP, but increased the survival rate ( P < 0.05). Conclusions HMGB1 may be involved in the process of myocardial apoptosis in sepsis, which leads to cardiac dysfunction. Sodium butyrate can reduce myocardial apoptosis in sepsis and ameliorate cardiac dysfunction.关键词
高迁移率族蛋白类/脓毒症/心肌凋亡/脑钠肽/正丁酸钠Key words
HMGB1 protein/ sepsis/ myocyte apoptosis/ BNP/ sodium butyrate分类
临床医学引用本文复制引用
徐海洲,章德文,沈美华,梅冰,何建..血清高迁移率族蛋白B1与脓毒症小鼠心肌细胞凋亡的相关性研究[J].武警医学,2011,22(8):688-692,5.基金项目
上海市公共卫生优秀学科带头人基金(08GWD25) （08GWD25）
