林业科学2011，Vol.47Issue(6)：42-47,6.
真菌诱导子促进白桦悬浮细胞三萜的积累
Accumulation of Triterpenoids in Betula platyphylla Suspension Culture Induced by a Fungal Elicitor
摘要
Abstract
Endophytic fungus of Phomopsis isolated from birch(Betula platyphylla) bark can be used as an elicitor to induce accumulation of triterpenoids in birch cell suspension culture. The change of dry weight and triterpenoids content of birch suspension cell were investigated at early growth phase, exponential growth phase and late growth phase after a fungal elicitor of 40, 100, and 400 (xg-mL"' concentration was added to birch suspension cells. Results showed that different induction schemes all promoted accumulation of triterpenoids, while the treatments reduced the dry weight of birch cells. Suspension cells at exponential growth phase were most easy to be induced, and 40 ng-mL"' fungal elicitor enhanced the triterpenoids content to 29. 47 mg-g"1, increasing by 789$j than that of the control. We investigated changes in pH and conductivity of the medium, and the activity of phenylalanine ammonialyase ( PAL) and peroxidase ( POD) under the optimal induction condition. Results showed that the fungal elicitor induced alkalinization of the medium and increased the conductivity, and significantly increased the activity of PAL and POD of birch suspension cells, which was 5. 70 and S. 74 fold as high as the control after 6 - 10 h of treatment, respectively. These results suggested that fungal elicitation induced defense reaction in birch suspension cell. General phenylpropanoid pathway and oxidative burst might involved in the triterpenoids accumulation induced by the fungal elicitor.关键词
白桦/真菌诱导子/三萜/悬浮细胞培养Key words
Betula platyphylla/ fungal elicitor/ triterpenoids/ suspension culture分类
农业科技引用本文复制引用
翟俏丽,范桂枝,詹亚光..真菌诱导子促进白桦悬浮细胞三萜的积累[J].林业科学,2011,47(6):42-47,6.基金项目
东北林业大学研究生科技创新项目(000-41100610) （000-41100610）
中央高校基本科研业务专项资金项目(DL09BA05) （DL09BA05）
黑龙江省自然科学基金重点项目(ZD200918) （ZD200918）
国家自然科学基金项目(31070531). （31070531）
