动物营养学报2011，Vol.23Issue(8)：1303-1308,6.DOI:10.3969/j.issn.1006-267x.2011.08.008
小肽转运载体2在奶牛乳腺小肽摄取中的作用研究
Role of Oligopeptide Transporter 2 in Bovine Mammary Gland Phenylalanine Dipeptide Uptake
摘要
Abstract
This experiment was conducted to study the role of oligopeptide transporter 2 in small peptides uptake and milk protein synthesis in bovine mammary gland. Different doses of Phe dipeptide (0 and 11. 7 |xg/mL)and DEPC (0, 0.01, 0.1, 0. 5 and 1 mmol/L) were added to the culture medium of bovine mammary gland tissues. After incubated in the experimental medium, mammary tissues and medium were collected and used for gene expression and milk protein determination, respectively. The results showed that l) Phe dipeptide increased oligopeptide transporter 2 and αsl -casein gene expression and milk protein quantity in the medium (P<0.05); 2) with increasing of DEPC dose, αsl-casein gene mRNA level (P <0. 01) and synthesis of milk protein (P < 0.05) were decreased; 3) treatment with 0.5 mmol/L DEPC significantly decreased a,,-casein gene expression (P < 0. 05) and synthesis of milk protein (P < 0. 01) in Phe dipeptide group, and synthesis of milk protein (P <0.05) in free Phe group, but had no effect on αsl-casein gene mRNA level (P >0.05) in free Phe group. These results indicate that Phe dipeptide can be used for synthesis of milk protein by bovine mammary gland while PepT2 may play an important role in small peptides uptake by bovine mammary gland. [Chinese Journal of Animal Nutrition, 2011, 23(8):1303-1308]关键词
二肽/PepT 2/酪蛋白/DEPC/奶牛乳腺组织Key words
dipeptides/ PepT2/ casein/ DEPC/ bovine mammary gland tissues分类
农业科技引用本文复制引用
周苗苗,吴跃明,刘红云,赵珂,刘建新..小肽转运载体2在奶牛乳腺小肽摄取中的作用研究[J].动物营养学报,2011,23(8):1303-1308,6.基金项目
国家重点基础研究发展计划(2011CBI00801) （2011CBI00801）
浙江省自然科学基金(Z305036) （Z305036）
