中国草食动物2011,Vol.31Issue(4):9-12,4.
湖羊BMPR-IB、BMP15和GDF9基因的RFLP分析
RFLP Analysis on BMPR-IB, BMP15 and GDF9 Genes in Hu Sheep
摘要
Abstract
Selecting BMPR-1B,BMP15 and GDF9 as candidate genes for prolificacy trait of Hu sheep,their single nucleotide polymorphisms were genotyped in 53 Hu sheep by PCR-RFLP method. The results showed that there were only BB and +B genotypes on the FecB locus of BMPR-IB gene,and their frequency were 0.981 1 and 0.018 9,respectively. The B was absolutely preponderance allele, and its frequency was 0.990 6. The B4 mutation of BMP15 gene and G8 mutation of GDF9 gene were not found in the population studied. So,it was speculated that the FecB locus of BMPR-IB gene was a major gene for prolificacy trait of Hu sheep; but the BMP15 gene and GDF9 gene had to the little relationship to litter size of Hu sheep. And the results indicated simultaneously that the population of Hu sheep detected was a genetically highly homozygous valuable breed resource of sheep.关键词
湖羊/BMPR-IB/BMP15/GDF9/RFLKey words
Hu sheep/BMPR-1B/BMP15/GDF9/RFLP分类
农业科技引用本文复制引用
吴丽丽,赵晓枫,张立凡,陈哲,徐宁迎..湖羊BMPR-IB、BMP15和GDF9基因的RFLP分析[J].中国草食动物,2011,31(4):9-12,4.基金项目
浙江省科技厅重大专项(2007C12057) (2007C12057)