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湖羊BMPR-IB、BMP15和GDF9基因的RFLP分析

吴丽丽 赵晓枫 张立凡 陈哲 徐宁迎

中国草食动物2011,Vol.31Issue(4):9-12,4.
中国草食动物2011,Vol.31Issue(4):9-12,4.

湖羊BMPR-IB、BMP15和GDF9基因的RFLP分析

RFLP Analysis on BMPR-IB, BMP15 and GDF9 Genes in Hu Sheep

吴丽丽 1赵晓枫 2张立凡 2陈哲 2徐宁迎2

作者信息

  • 1. 海南大学农学院,海口570228
  • 2. 浙江大学动物科学学院,杭州310058
  • 折叠

摘要

Abstract

Selecting BMPR-1B,BMP15 and GDF9 as candidate genes for prolificacy trait of Hu sheep,their single nucleotide polymorphisms were genotyped in 53 Hu sheep by PCR-RFLP method. The results showed that there were only BB and +B genotypes on the FecB locus of BMPR-IB gene,and their frequency were 0.981 1 and 0.018 9,respectively. The B was absolutely preponderance allele, and its frequency was 0.990 6. The B4 mutation of BMP15 gene and G8 mutation of GDF9 gene were not found in the population studied. So,it was speculated that the FecB locus of BMPR-IB gene was a major gene for prolificacy trait of Hu sheep; but the BMP15 gene and GDF9 gene had to the little relationship to litter size of Hu sheep. And the results indicated simultaneously that the population of Hu sheep detected was a genetically highly homozygous valuable breed resource of sheep.

关键词

湖羊/BMPR-IB/BMP15/GDF9/RFL

Key words

Hu sheep/BMPR-1B/BMP15/GDF9/RFLP

分类

农业科技

引用本文复制引用

吴丽丽,赵晓枫,张立凡,陈哲,徐宁迎..湖羊BMPR-IB、BMP15和GDF9基因的RFLP分析[J].中国草食动物,2011,31(4):9-12,4.

基金项目

浙江省科技厅重大专项(2007C12057) (2007C12057)

中国草食动物

2095-3887

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