肿瘤防治研究Issue(9)：983-985,3.DOI:10.3971/j.issn.1000-8578.2011.09.003
IFN-γ对白血病细胞株FBL-3细胞生物学行为的影响
Effects of IFN-γ on Proliferation,Cell Cycle, Apoptosis and Invasion of Leukemia Cell Line FBL-3
陈香丽 1张王刚 2王连才 3郭建民 1张茵 1马肖容 2田玮2
作者信息
- 1. 450003郑州,河南省人民医院血液内科
- 2. 西安交通大学第二附属医院血液内科
- 3. 河南省人民医院肝胆外科
- 折叠
摘要
Abstract
Objective To investigate the effects of interferon-γ (IFN-γ) on proliferation,cell cycle,apopto-sis and invasion of mouse leukemia cell line FBL-3. Methods The leukemia cellular proliferation capacity was determined with MTT assay,cell cycle distribution and apoptotic rate were measured with flow cy-tometry and the invasion capacity was assayed in a Transwell cell culture chamber. Result (1)Low dose of IFN-γ (5 ng/ml) had no significant effects on proliferation of FBL-3 (P>0. 05). The survival rate of FBL-3 stayed up to about 80% after treated by IFN-γ (50~200 ng/ml) for 48 h while higher dose of IFN-γ (500 ng/ml) had significant suppressive effects (less than 50%) (P<0. 05); (2)The FBL-3 cell number at G0/G1 phase was increased and cell number at G2/M phase was relatively reduced after treatment, indicating that cells were arrested at G0/G1 phase by IFN-γ; (3) IFN-γ had promotive effects on ap-optosis and suppressive effects on cell invasion capacity of FBL-3 cells in a dose-dependent manner (P< 0. 05). Conclusion IFN-γ had definite effects against leukemia through suppressing proliferation, promoting apoptosis and decreasing cell invasion directly in a dose-dependent manner.关键词
白血病/IFN-γ/细胞周期/凋亡/侵袭Key words
Leukemia/ IFN-γ/ Cell cycle/ Apoptosis/ Invasion分类
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陈香丽,张王刚,王连才,郭建民,张茵,马肖容,田玮..IFN-γ对白血病细胞株FBL-3细胞生物学行为的影响[J].肿瘤防治研究,2011,(9):983-985,3.
