肿瘤防治研究Issue(9)：991-994,1065,5.DOI:10.3971/j.issn.1000-8578.2011.09.005
神经酰胺促胃癌SGC7901细胞凋亡的实验
Ceramide Promoting Apoptosis of SGC7901 Cell
摘要
Abstract
Objective To investigate the role of ceramide on apoptosis of human gastric cancer SGC7901 cells and its possible mechanism. Methods SGC7901 cells were incubated and treated with the different concentration of ceramide,DDP,DDP in combination with ceramide. Then the inhibitory effect,the apoptosis rate and the expressions of NF-κB,Bcl-2,Bax were detected by MTT assay,flow cytometry, immu-nocytochemistry staining and western blot assay. Results The proliferation of SGC7901 cells was inhibited by ceramide at the concentration of 2. 5 μmol/L compared with control group(P<0. 05). Ceramide in combination with DDP showed significant differences compared with ceramide and DDP group respectively (P<0.05),the q value at 24,48,72h was 1.05,1.01,0.99 respectively. Ceramide,DDP could induce apoptosis of SGC7901 ,the apoptosis rate of ceramide 5 μmol/L,DDP 2. 5 mg/L and Ceramied 5 μmnol/L in combination with DDP 2. 5 mg/L group were (39. 23 ± 1. 62) %, (47. 27 ± 1. 13) %, (50. 13 ± 2. 76) % respectively, which showed significant difference compared with control group[(18. 46 ± 1. 64)%](P< 0. 05). Ceramide in combination with DDP showed significant differences compared with ceramide and DDP group respectively (P<0. 05). The expressions of NF-kB,Bc1-2 were strong in SGC7901[(74.10 ± 2. 69)%,(69. 37 ±4. 54)%)],while Bax was weak[(24. 60 ±3. 73)]%,the expressions of NF-κB,Bcl-2 decreased in Ceramide 5 μmol/L,DDP 2. 5 mg/L group and Ceramide 5μmol/L in combination with DDP 2. 5 mg/L group(65. 13 ± 1. 71,62.17 ± 2. 12,44. 8 ± 3. 65; 57. 70 ± 2. 22,55. 13 ± 5. 77,37. 67 ± 2. 14), while Bax increased (33. 80 ± 1.10,35. 50 ± 2.27,51. 73 ± 3. 76) (P<0. 05). The ratio of Bcl-2/Bax decreased after treated with ceramide,DDP and ceramide in combination with DDP. There is positive correlation between the expressions of NF-κB and Bcl-2(Spearman's rho = 0. 9510,Prob > |t| =0. 0000). Conclusion Ceramide can induce apoptosis by decreasing the expression of NF-kB via changing the ratio of Bcl-2/Bax in gastric cancer SGC7901 cells.关键词
SGC7901细胞/神经酰胺/核因子κB/Bcl-2/BaxKey words
SGC7901 cells/ Ceramide / NF-κB/ Bcl-2/ Bax分类
医药卫生引用本文复制引用
刘莹,朱祖安,费素娟,刘磊,孙旻,张秋月..神经酰胺促胃癌SGC7901细胞凋亡的实验[J].肿瘤防治研究,2011,(9):991-994,1065,5.基金项目
江苏省教育厅自然科学基金资助项目(05KJD320234) （05KJD320234）
徐州医学院肿瘤生物治疗重点实验室开放课题资助项目(C0806) （C0806）
江苏省卫生厅科研课题资助项目(Z201016) （Z201016）
