肿瘤防治研究Issue(9)：1002-1005,4.DOI:10.3971/j.issn.1000-8578.2011.09.008
褪黑素对肺癌A549细胞诱导的血管内皮细胞增殖的影响
Effects of Melatonin on Endothelial Cell Proliferation Induced by Lung Cancer Cell
摘要
Abstract
Objective To explore the effect of melatonin on endothelial cells proliferation induced by lung cancer cells A549 using a coculture system in vitro and its mechanism. Methods Lung cancer cells were cocultured with endothelial cells(HUVECs) in transwell chamber culture system. The cells were divided randomly into four groups: C group,nM group(final concentration of melatonin in the medium, 1 nM/L), μM group( final concentration of melatonin in the medium, 1μM/L) and mM group( final concentration of melatonin in the medium, 1 mM/L). Brdu method was carried out to assay the proliferation of HUVECs when treated with melatonin in concentrations from 1 nmol/L to 1 mM/L( final concentration in the medium). Transwell chamber assay was applied to measure the migration of HUVECs,and ELISA assay was performed to detect the VEGF protein levels in tumor cells. Results At near physiological concentrations (1nM/L) .melatonin had no significant effect on cellular proliferation. However,melatonin in higher concentrations (1mM/L) significantly decreased cell proliferation, and the negative effect on HUVECs proliferation was enhanced with increasing melatonin concentrations. Pharmacologic concentrations of melato-nin(1mM/L) significantly suppressed the VEGF protein levels. Conclusion High concentrations of melatonin markedly reduces HUVECs proliferation and migration. The protective mechanism might associate with suppression of VEGF protein expression.关键词
褪黑素/人脐静脉内皮细胞/共培养/人血管内皮生长因子/抗增殖Key words
Melatonion/ Human umbilical vein endothelial cell/ Coculture/ Vascular endothelial growth factor(VEGF)/ Antiproliferation分类
医药卫生引用本文复制引用
彭兴春,余明华,骆志国,崔培林..褪黑素对肺癌A549细胞诱导的血管内皮细胞增殖的影响[J].肿瘤防治研究,2011,(9):1002-1005,4.基金项目
国家自然科学基金资助项目(30901745) （30901745）
