肿瘤防治研究Issue(9)：1006-1011,6.DOI:10.3971/j.issn.1000-8578.2011.09.009
Apogossypolone诱导前列腺癌PC-3细胞在体外的自噬
Apogossypolone Induces Autophagy of PC-3 Prostate Cancer Cells in vitro
袁青 1陈晓鹏 2黄晓峰 3穆士杰 2胡兴斌 2尹文 1张献清2
作者信息
- 1. 710032西安,第四军医大学西京医院急诊科
- 2. 710032西安,第四军医大学西京医院输血科
- 3. 第四军医大学基础医学部中心实验室
- 折叠
摘要
Abstract
Objective To study the growth inhibitation action of gossypol derivatives, ApoG2 on the pros-tatic cancer PC-3 cells, and make the primary study about their anti-tumor mechanism. Methods The MTT analytical method, AO staining,transmission electron microscope .flow cytometer and Western blot for measuring the apoptosis related proteins were used to study the growth inhibition and induction of au-tophagy roles of ApoG2 on the prostatic cancer PC-3 cells in vitro. Results When the concentration of ApoG2 was higher than 2. 5 μg/ml in solution, it had the obvious proliferation inhibition ability to the prostate cancer PC-3 cells in vitro,and it had the charcteristics of time and dose dependent. When ApoG2 was used for the treatment of PC-3 cell for 72 hours, the observation with AO staining and transmission electron microscope indicated that ApoG2 could obviously induce the autophagy of prostate cancer cells. The measurement results by the flow cytometer indicated that the apoptosis of prostate cancer cells induced by ApoG2 might be strengthened by the use of autophagic inhibitor 3-MA. The measurement of Bcl-2 expressions by the Western blot found that after ApoG2 at the concentration of 10. 0 μg/ml was used for treatment of prostate cancer cells for 48 hours, the expression level of Bcl-2 decreased. The Beclin 1 and LC-3 Ⅱ expressions in the tumor cells were obviously increased by immunohistochemistry method after ApoG2 treatment Conclusion ApoG2 has the same obvious anti-proliferation role to the prostatic cancer PC-3 cells in vitro and its mechanism is to mainly induce the autophagy of tumor cells and to secondarily induce their death, the inhibition of autophagy may promote the occurrence of death.关键词
ApoG2/人前列腺癌PC-3细胞/自噬/凋亡Key words
Apogossypolone/ Prostate cancer PC-3 cells/ Autophagy/ Apoptosis分类
医药卫生引用本文复制引用
袁青,陈晓鹏,黄晓峰,穆士杰,胡兴斌,尹文,张献清..Apogossypolone诱导前列腺癌PC-3细胞在体外的自噬[J].肿瘤防治研究,2011,(9):1006-1011,6.
