中国病理生理杂志2011，Vol.27Issue(11)：2077-2081,5.DOI:10.3969/j.issn.1000-4718.2011.11.006
PPARδ激动剂GW501516抑制ox-LDL或高糖诱导的人脐静脉内皮细胞凋亡
PPARδ agonist GW501516 inhibits ox-LDL-or high glucose-induced apoptosis in human umbilical vein endothelial cells
摘要
Abstract
AIM:To investigate the inhibitory effect of peroxisome proliferator - activated receptor 8 ( PPAR8) agonist GW501516 on the apoptosis induced by oxidized low-density lipoprotein(ox-LDL) or high concentration of glucose in human umbilical vein endothelial cells ( HUVECs). METHODS; Cell apoptosis was induced by ox - LDL or high concentration of glucose in HUVECs and was examined by flow cytometry. The HUVECs were treated with GW501516 at different concentrations. The viability of HUVECs was analyzed by MTT assay. RESULTS; The apoptosis rate of HUVECs treated with ox - LDL was 21. 3% , while those of HUVECs treated with ox - LDL combined with low, medium and high concentrations of GW501516 were 17.47% , 9. 72% and 3.94% , respectively. The apoptosis rate of HUVECs treated with glucose was 22. 60% , while those of HUVECs treated with glucose combined with different concentrations of GW501516 were 20. 23% , 17. 01% and 9. 38% , respectively. The results indicated that ox - LDL or glucose induced apoptosis of HUVECs and GW501516 decreased the apoptotic rates induced by ox - LDL or glucose in a dose - dependent manner. The results of MTT assay showed that glucose or ox - LDL decreased the viability of HUVECs and GW501516 attenuated the effect of glucose or ox - LDL on HUVECs. CONCLUSION; GW501516 inhibits the apoptotic effects of ox - LDL and glucose on HUVECs and increases the viability of the cells.关键词
过氧化物酶体增殖物激活受体δ/GW501516/人脐静脉内皮细胞/细胞凋亡Key words
Peroxisome proliferator - activated receptor 5/ GW501516/ Human umbilical vein endothelial cells/ Apoptosis分类
医药卫生引用本文复制引用
王静,王晓光,史君,王星,李春跃,赵静..PPARδ激动剂GW501516抑制ox-LDL或高糖诱导的人脐静脉内皮细胞凋亡[J].中国病理生理杂志,2011,27(11):2077-2081,5.基金项目
内蒙古医学院青年基金资助项目(No.NY2006QN007) （No.NY2006QN007）
