中国病理生理杂志2011，Vol.27Issue(11)：2160-2164,5.DOI:10.3969/j.issn.1000-4718.2011.11.023
氧化应激在高脂高盐饮食伴高糖饮水诱导的代谢综合征肾病大鼠中的作用
Effects of oxidative stress on high-fat, high-salt diet and sucrose solution-induced metabolic syndrome in nephropathy rats
摘要
Abstract
AIM; To establish a suitable animal model of nephropathy associated with metabolic syndrome (MS) induced by abnormal diet, and to investigate the effects of oxidalive stress on renal damage in MS rats. METHODS; Normal 7 -week -old male SD rats were randomly divided into 2 groups. The animals were fed with normal chow (control group, n = 10) or high - fat and high - salt diet plus 20% sucrose solution ( MS model group, n = 10) for 20 weeks. Systolic blood pressure (SBP) was measured monthly. The levels of blood glucose, serum and urinary creatinine (Cr) , total cholesterol (TC) , triglycerides (TG) , fasting insulin (Fins) , urinary protein, urinary albumin and urinary sodium were determined. Insulin resistance ( HOMA - IR), creatinine clearance ( Ccr) , urinary protein excretion ( UPE) , urinary albumin excretion (UAE) and urinary sodium excretion (USE) were calculated. Renal total -antioxidant capacity (T - AOC), inhibiting superoxide anion capacity (ISAC) , malondialdehyde (MDA) content, and antioxidant enzyme activity were measured. Renal protein expression of Cu/Zn - SOD, NADPH oxidase subunit P47pboxand p22pbox was detected by Western blotting. In addition, pathological changes of the kidney were observed with PAS and Masson staining,and degree of glomerulosclerosis (GS) and tubulointerstitial injury was evaluated. RESULTS; Compared with control rats, SBP, TC, TG, Fins, USE and UAE were increased in MS rats. Furthermore, the MS rats showed a significant elevation of renal MDA content, p47pbox protein expression and GS score, and reduction of T - AOC, ISAC, SOD activity, and Cu/Zn - SOD protein expression in the kidney. CONCLUSION; SD rats fed with abnormal diet produce a suitable animal model of MS nephropathy that mimics the major features of human MS. Oxidative stress caused by up - regulation of NADPH oxidase expression and down-regulation of SOD expression may be one of the mechanisms leading to MS renal damage.关键词
代谢综合征/肾病/氧化性应激/NADPH氧化酶/抗氧化酶Key words
Metabolic syndrome/ Nephrosis/ Oxidative stress/ NADPH oxidase/ Antioxidant enzyme分类
医药卫生引用本文复制引用
孔祥,张道友,张妍,吴海兵,李芳霞..氧化应激在高脂高盐饮食伴高糖饮水诱导的代谢综合征肾病大鼠中的作用[J].中国病理生理杂志,2011,27(11):2160-2164,5.基金项目
安徽省教育厅自然科学基金资助项目(No.KJ2010B466) （No.KJ2010B466）
安徽省高等学校省级优秀青年人才基金资助项目(No.2009SQR184) （No.2009SQR184）
安徽省肾脏病学会佳林豪科研基金资助项目(No.AHJLH 2008008) （No.AHJLH 2008008）
