中国病理生理杂志2011，Vol.27Issue(11)：2175-2179,5.DOI:10.3969/j.issn.1000-4718.2011.11.026
清胰汤对L-精氨酸诱发的重症急性胰腺炎小鼠胰腺p-STAT3表达的影响
Effect of Qingyi decoction on p-STAT3 expression in pancreas of mice with severe acute pancreatitis induced by L-arginine
摘要
Abstract
AIM: To explore the role of STAT3 signaling pathway in acute pancreatitis induced by L - arginine, and the mechanisms of Qingyi decoction(QYD) treatment on severe acute pancreatitis (SAP). METHODS: The Kunming mice were randomly divided into 3 groups (n = 10): control group, SAP group and QYD treatment group (SAP + QYD). The mice in SAP group and SAP + QYD group were intraperitoneally injected with 20% L - arginine (3 g/kg, bid). The mice in SAP + QYD group were also administered intragastrically with QYD( 10 ml/kg) 30 min after the second injection of 20% L - arginine and twice a day for the following 2 days. The mice were anesthetized and sacrificed 72 h after SAP induction. The activity of amylase was measured in serum, the relative pancreatic weight was assayed, and the activity of myeloperoxidase (MPO) was analyzed to evaluate the neutrophil infiltration in lung tissues. The morphology of pancreas and lung was observed. The protein of pancreas was extracted to detect the expression of p - STAT3 by Western blotting. The mRNA expression of monocyte chemoattractant protein - 1 ( MCP - 1 ) was determined by real - time PCR. RESULTS: Compared with control group at 72 h, L - arginine induced SAP with increased serum amylase activity, pancreatic wet weight ratio and MPO activity in lung tissues (P <0. 05). In SAP + QYD group, the activity of amylase, pancreatic wet weight ratio and MPO levels were significantly lower than those in SAP group ( P < 0. 01). Compared with control group at 72 h, the pancreas and lung were obvious injured, the protein level of p - STAT3 and mRNA expression of MCP - 1 in pancreas tissues increased significantly in SAP group. Compared with SAP group, the pathologic damage of the pancreas and lung tissues, the protein level of p - STAT3 and mRNA expression of MCP - 1 in pancreas were significantly reduced in SAP + QYD group. CONCLUSION: The expression of p - STAT3 in pancreas increases in the mice with SAP induced by L - arginine. The activation of STAT3 may take part in the development of SAP. Inhibition of STAT3 activation in pancreas is one of the mechanisms of QYD treatment for SAP.关键词
信号转导和转录激活因子3/急性胰腺炎/清胰汤Key words
Signal transducer and activator of transcription 3/ Acute pancreatitis/ Qingyit decoction分类
医药卫生引用本文复制引用
张晓芹,贾晓云,李涛,李保兰,史迎莉,张红..清胰汤对L-精氨酸诱发的重症急性胰腺炎小鼠胰腺p-STAT3表达的影响[J].中国病理生理杂志,2011,27(11):2175-2179,5.基金项目
陕西省自然科学研究基础项目(No.2010JM4023) （No.2010JM4023）
咸阳市自然科学研究项目[No.2010K14-02(6)] （6）
