中国病理生理杂志2011，Vol.27Issue(11)：2194-2198,5.DOI:10.3969/j.issn.1000-4718.2011.11.030
阻塞性黄疸对肠上皮细胞氯离子分泌影响的研究
Effects of obstructive jaundice on enterocyte chloridion secretion in rats
摘要
Abstract
AIM: To investigate the effects of obstructive jaundice ( OJ) on enterocyte chloridion secretion in rats. METHODS; The OJ model of rats was set up. The rats were divided into 0J1 group and 0J2 group randomly. The animals in 0J1 group were sacrificed by exsanguination 7 days after operation and the rats in 0J2 group were sacrificed 14 days after operation. The Cl - concentration in peripheral blood was detected. The epithelial cells in the terminal ileum mucosas were cultured in vitro. The concentration f)f intracellular Cl- was detected by fluorospectrophotometry. The Cl- current was measured by whole - cell patch clamp experiments. Western blotting was used to examine the change of voltage gated Cl - channel 2 protein ( C1C - 2) and the images were analyzed by image analysis system and statistical software quantitatively. RESULTS; The serum Cl" concentration in 0J1 group and OJ2 group were obviously lower than that in control group [(88. 67 ±4.68) mmol/L and (82.01 ±6.25) mmol/L vs (95.57±7.51) mmol/L, both P<0. 05]. The intracellular Cl- relative concentrations in OJ1 group and 0J2 group were higher than that in control group ( 3. 14 ± 0. 38 and 3. 55 ± 0.47 vs 2.69 ± 0.41, both P < 0.05 ). The Cl" current of normal epithelial cells displayed outward rectification, and transformed negative value into positive value following cell membrane depolarization. The Cl~ current was ( -15.45±7.56) pA/pFand (5. 85 ±0. 81) pA/pF when voltage was - 80 mV and 80 mV. The ranges of upgrade of absolute values of average electric current density in OJ groups were lower than those in control group (all P < 0. 05). Western blotting analysis showed that the C1C - 2 protein generated a broad band about 90 kD. The average gradations of Western blotting images were lower in OJ groups than that in control group (0. 20 ±0. 04 and 0. 19 ±0. 06 vs 0. 27 ±0. 06, both P < 0.05). However, no difference between the 2 OJ groups was observed ( P > 0. 05 ). CONCLUSION: The chloridion secretion of intestinal epithelium is restrained in rats with OJ. The concentration gradient between exterior and interior of epithelial cells is decreased, and the Cl' outward current is reduced. All of above are concerned with downregulation of C1C -2 protein in cell membrane.关键词
黄疸,梗塞性/肠细胞/氯化物通道Key words
Jaundice, obstructive/ Enterocytes/ Chloride channels分类
医药卫生引用本文复制引用
陈振勇,戴红梅,杨鹏,王延刚,黄文广,冯贤松..阻塞性黄疸对肠上皮细胞氯离子分泌影响的研究[J].中国病理生理杂志,2011,27(11):2194-2198,5.基金项目
湖北省自然科学基金资助项目(No.2010CDB08005) （No.2010CDB08005）
