色谱2011，Vol.29Issue(6)：523-534,12.DOI:10.3724/SP.J.1123.2011.00523
液相色谱-串联质谱法测定谷物类饲料中的41种激素
Determination of 41 hormones in cereal feeds by liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was developed for the determination of 10 androgens, 11 progesterones, 10 glucocorticoids, 5 estrogens and 5 resorcylic acid lactones in cereal feeds by liquid chroma-tography-tandem mass spectrometry (LC-MS/MS). Ethyl acetate was used to extract the sample , and the mixed QuEChERS sorbents were dispersed for purification. The extract was separated by a Clg column and detected in positive or negative multiple reaction monitoring (MRM) mode. With the optimal conditions, the linear ranges of 41 hormones were 5. 0 - 200. 0 |xg/kg with the correlation coefficients above 0. 99 and the limits of quantification (LOQs, S/N 3? 10) were 0. 123 -2.72 jig/kg. The method validation was carried out at three spiking levels, and the recoveries were 70.3% - 118. 1% for soybean meals with the relative standard deviations ( RSDs) of 0. 82% - 19. 5%, and 76. 1% - 122. 8% for com meals with the RSD of 1. 3% - 15. 0%. This method is simple, fast and credible, can be applied to simultaneous screening and determination of estrogens, progesterones, androgens, glucocorticoids and resorcylic acid lactones belonging to the illegal additive of hormones in cereal feeds.关键词
液相色谱-串联质谱/雄性激素/孕激素/糖皮质激素/雌性激素/二羟基苯甲酸内酯/谷物类饲料Key words
liquid chromatography-tandem mass spectrometry (LC-MS/MS)/ androgen/ progesterone / glucocorticoid/ estrogen/ resorcylic acid lactones/ cereal feeds分类
化学化工引用本文复制引用
张毅,蓝芳,张峰,沈金灿,储晓刚..液相色谱-串联质谱法测定谷物类饲料中的41种激素[J].色谱,2011,29(6):523-534,12.基金项目
国家质检总局科技计划项目(2008IK167). （2008IK167）
