色谱2011，Vol.29Issue(6)：558-562,5.DOI:10.3724/SP.J.1123.2011.00558
鹿茸保健品中11种性激素的气相色谱-串联质谱法分析
Simultaneous determination of eleven sex hormones in antler velvet health products by gas chromatography-tandem mass spectrometry
摘要
Abstract
A method for the simultaneous determination of 11 sex hormones in antler velvet health products by gas chromatography-tandem mass spectrometry ( GC-MS/MS) was developed. The sex hormones in antler velvet were enriched and purified by solid phase extraction and derivatized with heptafluorobutyric acid anhydride (HFBA). A DB-5 column (30 m xO. 25 mm, 0. 25 μm) with nonlinear gradient program was used in GC separation. The sex hormones were determined in the multiple reaction monitoring mode. The method realized the complete separation of 11 sex hormones. The limits of detection of this method were from 1.0 to 5. 0 (μg/kg for the 11 sex hormones. The correlation coefficients were between 0. 991 6 and 0. 999 9. The recoveries were in the range of 67. 4% -99. 1% with relative standard deviations (RSDs) of 2. 6% - 13%. This method is accurate and reliable for the determination of the sex hormones in antler velvet health products.关键词
气相色谱-串联质谱/性激素/鹿茸/保健品Key words
gas chromatography-tandem mass spectrometry (GC-MS/MS)/ sex hormones/ antler velvet/ health products分类
化学化工引用本文复制引用
芦春梅,王明泰,牟俊,卢利军,周晓..鹿茸保健品中11种性激素的气相色谱-串联质谱法分析[J].色谱,2011,29(6):558-562,5.基金项目
国家质检总局科研项目(项目编号:2007IK157). （项目编号:2007IK157）
