中国医科大学学报2011，Vol.40Issue(10)：902-905,4.DOI:21-1227/R.20111019.1722.003
纳米羟基磷灰石/胶原蛋白复合骨结合VEGF修复骨缺损的实验研究
Experimental Research of Nano-hydroxyapatite/collagen Materials Combined with Vascular Endothelial Growth Factor for the Repair of Bone Defects
顾晓东 1梁庆威1
作者信息
- 1. 中国医科大学附属第一医院骨科,沈阳110001
- 折叠
摘要
Abstract
Objective To investigate the effect of nano-hydroxyapatite/collagen (NHAC) materials combined with variant doses of vascular endothelial growth factor (VEGF) for the repair of bone defects. Methods Nano-hydroxyapatite powder and collagen powder were mixed in the ratio of 8:2 and prepared into NHAC artificial bone. The mixed powder was mixed with 10 ng, 100 ng and 300 ng of VEGF respectively in the ratio of 1 '■ 1.8, and were then prepared into NHAC/VEGF artificial bone. Thirty healthy rats whose radiuses were cut off 0.5 cm were divided into five groups (twelve radiuses in each group). Groups in which bone defects of rats were repaired with NHAC/10 ngVEGF, NHAC/100 ngVEGF and NHAC/300 ngVEGF artificial bones were the experimental groups. The NHAC group and the blank group were the control groups. The vascularizan'on of artificial bone implanted into the defect area and new-bone formation were evaluated by histologies] and immunohistochemical analysis at 2,4 and 8 weeks postoperatively. Results The new bone formation,molding histological score and blood vessel count at each time point of NHAC/300 ngVEGF group were significantly higher compared to those of the other groups (P < 0.05). Blood vessel counts in the experimental groups reached the maximum at 4 week .while the NHAC group at 8 week. Conclusion VEGF is beneficial for promoting early vascularization of the NHAC and improves the repair of bone defects,and the blood vessel count and new bone formation increase as VEGF increases.关键词
纳米羟基磷灰石/胶原蛋白/血管内皮生长因子/骨缺损Key words
nano-hydroxyapatite/collagen/vascular endothelial growth factor/bone defects分类
医药卫生引用本文复制引用
顾晓东,梁庆威..纳米羟基磷灰石/胶原蛋白复合骨结合VEGF修复骨缺损的实验研究[J].中国医科大学学报,2011,40(10):902-905,4.
