解放军医学杂志2011，Vol.36Issue(7)：714-716,3.
气体信号分子硫化氢对高脂饲养大鼠肝脏脂代谢的调节作用
Effects of hydrogen sulfide on lipid metabolism in liver of high-fat-fed rats
关望 1刘文莉 2王海英 2关岚 2赵晓东 2赵斌3
作者信息
- 1. 100035北京,北京积水潭医院干部科
- 2. 100035北京,北京积水潭医院急诊科
- 3. 100048北京,解放军总医院第一附属医院急救部
- 折叠
摘要
Abstract
Objective To investigate the effects of hydrogen sulfide on lipid metabolism in liver of high-fat-fed (H-FD) rats. Methods Twenty-four male SD rats were randomly divided into control group, H-FD group and H-FD+NaHS group (8 each). Rats were sacrificed after feeding for eight weeks, and hepatic tissue was obtained and frozen sectioned. They were stained with oil red O, Philipin and HE. The oil red O and Philipin-dyed pictures were measured by image analysis system. Results Oil red O staining showed that the cell shape and structure of hepatic tissue were normal in control group, and massive diffuse lipid deposition and damaged structure in hepatic lobule were found in H-FD group. The lipid deposition decreased significantly, and the damage of hepatic lobule also ameliorated, in H-FD+NaHS group (5818. 79+297. 45) compared with H-FD group (62612. 70±756. 46, P<0. 01). Philipin staining showed that the fluorescence signal of total cholesterol was negative in control group, and it was positive in H-FD group where the signal was strong and well distributed. The fluorescence intensity of total cholesterol was significantly weakened in H-FD+NaHS group (52. 13 ±3. 70) compared with H-FD group (201. 21+3.18, P<0.01). Macroscopic examination with HE staining showed that the cell shape and structure of hepatic tissue were normal in control group; the hepatocytes contained big lipid droplets, becoming swollen and rounded with obviously injured central area of hepatic lobule in H-FD group; while the lipid droplets in hepatocytes was distinctly decreased in H-FD+NaHS group, and the shape and structure of hepatocytes recovered to certain extent. Conclusion Exogenous hydrogen sulfide might show a novel regulatory effect on abnormality of lipid metabolism in liver of high-fat-fed rats.关键词
硫化氢/脂代谢障碍/胆固醇Key words
hydrogen sulfide/ lipid metabolism disorders/ cholesterol分类
医药卫生引用本文复制引用
关望,刘文莉,王海英,关岚,赵晓东,赵斌..气体信号分子硫化氢对高脂饲养大鼠肝脏脂代谢的调节作用[J].解放军医学杂志,2011,36(7):714-716,3.
