中国药理学与毒理学杂志2011，Vol.25Issue(5)：419-424,6.DOI:10.3867/j.issn.1000-3002.2011.05.002
硫化氢通过抗氧化作用改善脑缺氧导致的小鼠空间记忆障碍
Hydrogen sulfide attenuates spatial memory disorder induced by cerebral anoxia via antioxidation in mice
摘要
Abstract
OBJECTIVE To investigate effects of exogenous hydrogen sulfide ( H2 S) on the spatial memory disorder induced by cerebral anoxia in mice and explore related mechanism.METHODS Sodium nitrite (NaNO2) 120 mg·kg-1 was sc given to mice for4 d in model group.Sodium hydrosulfide (NaHS) 1 mg·kg-1 was ip given and NaNO2 120 mg·kg-1 simultaneously was sc given to mice for4 d in NaHS group.All drugs were given to mice immediately after Morris water maze experiment every day and escape latency.The number of crossings over the target area (NCTA) and search time in target quadrant (STTQ) were recorded.The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) level in the brain was determined with colorimetry.The morphological alterations in hippocampus slices were assessed by microscope.RESULTS On the third and fourth days in Morris water maze experiment,compared with ( 16.1 ±9.6)s and ( 11.1 ±6.2)s in normal control group,the escape latency in model group was longer,(26.0 ±7.3)s(P＜0.05) and (23.3 ±8.7)s(P＜0.01).On the fifth day,compared with 7.2 ± 1.6 and (28 ± 8) s in normal control group NCTA and STTQ in model group were 4.1 ± 1.9 and (20 ± 8 ) s ( P ＜ 0.05 ),and they were obviously less.Compared with normal control group,SOD activity and M DA content of mice in model group were reduced by 12.6％ (P ＜ 0.01 ) and increased by 43.9％ (P ＜ 0.01 ),respectively.The neuron degenerative changes including karyopyknosis,dark cytoplasm and irregular pyramidal layer were observed in model group.On the third and fourth day,compared with model group,the escape latency in NaHS group was shorter,(17.9 ±7.0)s and (15.8 ±8.5)s (P＜0.05).Compared with model group,NCTA and STTQ in NaHS group increased to 6.7 ± 2.5 and ( 30 ± 9) s ( P ＜ 0.01 ).SOD activity and MDA content in NaHS group were increased by 8.9％ ( P ＜ 0.05 ) and reduced by 29.6％ ( P ＜ 0.01 ),respectively.Neuron degeneration was significantly attenuated in NaHS group (P ＜ 0.01 ).CONCLUSION NaHS can attenuate the spatial memory disorder induced by cerebral anoxia and the mechanism may be related to the antioxidation effect and alleviation of neuron damage of H2S.关键词
亚硝酸钠/硫化氢/学习记忆/神经元变性/海马/抗氧化作用Key words
Key words: sodium nitrite/hydrogen sulfide/learning and memory/neuron degeneration/hippocampus/antioxidation分类
医药卫生引用本文复制引用
武玉清,朱杨子,韩丹,张振,芮龙洁,黄河..硫化氢通过抗氧化作用改善脑缺氧导致的小鼠空间记忆障碍[J].中国药理学与毒理学杂志,2011,25(5):419-424,6.基金项目
Foundation item:The project supported by National Natural Science Foundation of China (81070889) （81070889）
National Natural Science Foundation of China(81171013) （81171013）
Key Subject of Provincial Colleges and Universities Natural Science Foundation of Jiangsu Province(10KJA320052) （10KJA320052）
and College Student's Innovation Practice of Jiangsu Province (2010797)国家自然科学基金资助项目(81070889) （2010797）
国家自然科学基金资助项目(81171013) （81171013）
江苏省高校自然科学基金重大项目资助项目(10KJA320052) （10KJA320052）
江苏省高等学校大学生实践创新训练计划资助项目(2010797) （2010797）
