厦门大学学报（自然科学版）2011，Vol.50Issue(4)：759-764,6.
拟穴青蟹HSP70基因的克隆与组织表达
Molecular Cloning and Tissue Expression of HSP 70 from the Mud Crab, Scylla paramamosain
摘要
Abstract
Heat shock protein 70 (HSP 70) is an in evolutionarily highly conserved protein, with a variety of biological functions. A full-length cDNA of HSP 70 was cloned from Scylla paramamosain using RACE and gene cloning method in this study. The full-length HSP 70 cDNA was 2 189 bp,including a 5'untranslated region (UTR) of 110 bp.a 3'UTR of 126 bp,and an open reading frame (ORF) of 1 953 bp nucleotides. The ORF encoded a polypeptide of 650 amino acids with calculated molecular mass of 71. 2 ku and theoretical point of 5. 38. Amino acid sequence alignment showed the S. Paramamosain HSP70 had high homology with that in some model animals. It suggested HSP70 was highly conservative. Phylogenetic tree based on comparison of HSP70 proteins, was consistent with the evolution relationship. The tissue expressions in 8 different tissues were tested by RT-PCR. The results showed that the expected PCR products were amplified from every tested tissues. It suggested that HSP 70 can be constitutively expressed in S. Paramamosain. The highest expression of HSP 70 was found in ovary,the following was in brain,this was possibly related with the functions of HSP 70 .关键词
拟穴青蟹/HSP 70/克隆/组织表达Key words
Scylla paramamosain /HSP 70/cloning/tissue expression分类
生物科学引用本文复制引用
李文杏,刘元婧,叶海辉,黄辉洋,李少菁..拟穴青蟹HSP70基因的克隆与组织表达[J].厦门大学学报（自然科学版）,2011,50(4):759-764,6.基金项目
国家自然科学基金项目(40406030 （）
41076081) （）
