南京医科大学学报（自然科学版）2011，Vol.31Issue(9)：1261-1265,5.
褐藻糖胶抑制人肝癌细胞HepG2增殖的机制
Mechanism of inhibiting the proliferation of the human hepatoma HepG2 cells by fucoidan
摘要
Abstract
Objective:To explore the effect and the related mechanisms of fucoidan on hepatoma cell proliferation. Methods;Proliferation inhibition rate of hepatoma HepG2 cells was measured by MTT. A variety of dosage of fucoidan (0 μg/ml, 10 μg/ml, 100 μg/ml and 500 μ/ml) was treated to HepG2 cells. The morphology changes of the cells were observed under microscopy. Apoptosis was detected by Hoechst 33258 staining and DNA Ladder analysis. CyclinDl and topoisomerase Ⅱα (TopoⅡα) were as the proliferation biomarker. Their protein expression levels were examined by Western blot. Results;Fucoidan inhibited HepC2 cells proliferation in a dose-dependent manner. There was a remarkable morphological change when cells were treated with 500 μg/ml of fucoidan. Apoptosis was occurred when the cells were incubated with 100 μg/ml and 500 μg/ml of fucoidan. In addition,fucoidan also suppressed the expression of cyclinDl and TopoIIa in HepC2 cells. Conclusion;Fucoidan can inhibit the proliferation of HepC2 cells and induce apoptosis. Meanwhile,fucoidan also has the ability to suppress the protein expression of cyclinDl and topollot,which may be involved in the mechanisms of inhibiting hepatoma cells proliferation.关键词
褐藻糖胶/人肝癌细胞HepG2/凋亡/cyclinD1/TopoⅡαKey words
fucoidan/human hepatoma cell line HepG2/apoptosis/cyclinDl/TopoⅡα分类
医药卫生引用本文复制引用
李小婷,殷辰俞,孟徐莲,段颉,孔秋月,冯晴..褐藻糖胶抑制人肝癌细胞HepG2增殖的机制[J].南京医科大学学报（自然科学版）,2011,31(9):1261-1265,5.基金项目
国家自然科学基金面上项目(30972479)和江苏省自然科学基金(BK2009421) （30972479）
