昆明医学院学报2011，Vol.32Issue(7)：40-46,7.
环孢霉素A联合脾内肝细胞移植治疗急性肝衰竭的实验研究
Therapeutic Effects of CsA Combined with Hepatocyte Intrasplenic Transplantation on Acute Hepatic Failure
摘要
Abstract
Objective To evaluate the therapeutic effects of CsA combined with allogenic hepatocyte through intrasplenic transplantation on rats with acute hepatic failure. Methods Acute hepatic failure (AHF) rats were induced by 350 mg/kg thioacetamide ( TAA) and randomly divided into 3 groups 24 h after intoxication. Rats in Group I received 1ml of approximately 2 X 107 hepatocytes suspension and CsA 10 mg/(kg.d)t through intrasplenic injection and intramuscular injection. Rats in Group II only received 1 mL of approximately 2 x 107 hepatocytes suspension. Rats in Group ID as control received 1 ml of normal saline. The survival rate, hepatic biochemical function and pathological changes of transplanted hepatocytes and liver of all rats were observed. Results One-week survival rate in group I and group H was higher than that of group ID (64.7% val2.5%, F<0.01; and 56.3% vs 12.5%, P<0.01, respectively), while there was no significant difference in one-week survival rate between group I and group II (64.7% vs 56.3%, P>0.05). Hepatic function and liver pathological state of rats were improved significantly in group I and group II , especiall in group I. Conclusion CsA combined with allogenic hepatocyte transplantation can effectively treat rats with acute hepatic failure, improve the survival rate of rats, and ameliorate hepatic function and hepatic pathological state.关键词
肝细胞移植/急性肝衰竭/硫代乙酰胺/环孢霉素AKey words
Hepatocyte transplantation/ Acute liver failure/ Thioacetamide/ Cyclosporine A分类
临床医学引用本文复制引用
李武,戴虹,何贵清,黄学惠,陈一晖,王超秀..环孢霉素A联合脾内肝细胞移植治疗急性肝衰竭的实验研究[J].昆明医学院学报,2011,32(7):40-46,7.基金项目
云南省教育厅科研基金资助项目(08C0131) （08C0131）
