动物营养学报2011，Vol.23Issue(7)：1140-1146,7.DOI:10.3969/j.issn.1006-267x.2011.07.011
大豆凝集素对鲤鱼肠道上皮细胞结构和功能的影响
The Influence of Soybean Agglutinin on the Structure and Function of Carp Intestinal Epithelial Cells
摘要
Abstract
This experiment was conducted to study the influence of soybean agglutinin (SB A) on the structure and function of carp intestinal epithelial cells in primary culture. The carp intestinal epithelial cells in primary culture were cultured in 24-well plate after 72 h, then changed into the DMEM culture medium which contained 0, 50, 75, 100, 150 and 200 μg/mL purified SBA, respectively. The samples were collected to measure the indices after 72 h SBA culture. The results showed that the activities of lactate dehydrogenase (LDH) and glutamic-pyruvic transaminase (GPT) in culture medium of carp intestinal epithelial cells were significantly enhanced by SBA (P <0. 01) , but the influence on glutamic-oxalacetic transaminase (GOT) activity in the culture medium didn't reach significant level (P > 0. 05). The results of cell morphological observation showed that the cell shape was obviously changed by SBA, and it was changed from ellipse to long strip or irregular shape and the number of dead cells in culture medium increased. Meanwhile, the SBA could significantly increase the methyl thiazolyl tetrazolium optical density (MTT OD) value and protein content (P <0. 05 or P < 0. 01) in carp intestinal epithelial cells, and significantly enhance the activities of GOP and GPT in cells and ammonia concentration in culture medium (P <0.05 or P<0.01), but significantly decrease the activities of alkaline phosphatase (AKP) and Na+ ,K + -ATPase in cells (P <0. 01). These results indicate that SBA can damage the normal structure of cells, promote the proliferation and apoptosis of cells, restrain cell differentiation and accelerate the protein metabolism for carp intestinal epithelial cells.关键词
大豆凝集素/鲤鱼/肠道上皮细胞/结构/功能Key words
soybean agglutinin/ carp intestinal epithelial cells/ structure/ function分类
农业科技引用本文复制引用
冯琳,姜俊,刘扬,胡凯,姜维丹,周小秋..大豆凝集素对鲤鱼肠道上皮细胞结构和功能的影响[J].动物营养学报,2011,23(7):1140-1146,7.基金项目
公益性行业(农业)科研专项(201003020)经费资助 （农业）
