南京医科大学学报（自然科学版）2011，Vol.31Issue(7)：925-929,5.
稳定表达GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系的建立
Establishment of a stable GFP-C-Daam1 MDA-MB-231 breast cancer cell line
摘要
Abstract
Objective:To establish a MDA-M8-231 human breast cancer cell line stably express GFP-C-Daaml, and to investigate the influence of Daaml on the mobility of breast cancer cells. Methods;The lentivrial vectors containing GFP or GFP-C-Daaml were constructed and infected human breast cancer cells MDA-MB-231. MDA-MB-231 cells were screened by puromycin and stable expression of GFP or GFP-C-Daaml was confirmed by Western blotting. Boyden chamber assay was used to test the intrinsic motility of the cells. ResultS;The cell lines stably expressing GFP or GFP-C-Daaml were established successfully by the lentivrial infection. The GFP-C-Daaml expressing cells showed greater intrinsic motility, suggesting the products of the interesting gene were active and functional. Conclusion;MDA-MB-231 breast cancer cell lines stably expressing GFP or GFP-C-Daaml were successfully established using lentiviral methods. These cell lines can be used as model cells for further study of active Daaml targeting.关键词
C-Daam1/MDA-MB-231细胞/基因转染/稳定表达/细胞迁移Key words
C-Daaml/ MDA-MB-231 cell/ gene transfection/ stable expression/ cell migration分类
医药卫生引用本文复制引用
李卫星,杜军,胡圳圳,田寅辉,刘皎婧,朱一超..稳定表达GFP-C-Daam1的MDA-MB-231人乳腺癌细胞系的建立[J].南京医科大学学报（自然科学版）,2011,31(7):925-929,5.基金项目
国家自然科学基金资助(30872926),江苏省博士后科研资助(0902023C),南京医科大学科技发展基金重点项目(2010NJMUZ35) （30872926）
