南京医科大学学报（自然科学版）2011，Vol.31Issue(7)：945-950,6.
瘦素干扰他莫昔芬抑制乳腺癌细胞MCF-7增殖作用的机制
Mechanism of leptin inhibiting the growth effect of antiestrogen tamoxifen in MCF-7 breast cancer cells
摘要
Abstract
Objective;To identify the expression of leptin receptor in breast cancer cell line MCF-7, and to explore the effects and mechanisms of leptin on the anti-proliferative effect of tamoxifen on MCF-7. Methods:The expression of leptin receptor was detected by immunofluorescence and Western blot. After treated with 100 ng/ml of leptin and 1 000 nmol/L of tamoxifen (TAM), cell proliferation and the levels of estrogen receptor a(Erot) in MCF-7 were examined. Plasmid pERE-LUC which containing estrogen response element (ERE) and reporter gene luciferase was constructed and transfected into MCF-7 cells, the expression of luciferase was detected to reflect the effects of leptin on the transcription of Era. Results;Leptin receptor was positively expressed in MCF-7 cells. Leptin weaken the inhibition of TAM on MCF-7 proliferation by upreglulating the transcription of Era. Conclusion;Leptin could interfere with the inhibition of TAM on breast cancer cells, it may contribute to the efficacy of tamoxifen treatment for breast cancer patients. .关键词
乳腺癌/瘦素/他莫昔芬/雌激素受体αKey words
breast cancer/ leptin/ tamoxifen/ Era分类
医药卫生引用本文复制引用
朱婷婷,赵万,王凯,王蓉,殷咏梅..瘦素干扰他莫昔芬抑制乳腺癌细胞MCF-7增殖作用的机制[J].南京医科大学学报（自然科学版）,2011,31(7):945-950,6.基金项目
江苏省自然科学基金(BK2008477),人事部留学回国人员基金,江苏省卫生厅面上项目(H201001),2010年江苏省教育厅“青蓝工程”,2010年江苏省“六大人才高峰”,江苏省卫生厅开放课题(XK200904) （BK2008477）
