南京医科大学学报（自然科学版）2011，Vol.31Issue(7)：951-955,1006,6.
5-氟尿嘧啶-Fe/Fe3O4磁性脂质体的制备及其表征
Preparation and characterization of 5-fluorouracil-Fe/Fe3O4 magnetic liposome
摘要
Abstract
Objective:To prepare 5-fluorouracil (5-FU) magnetic liposome with Fe/Fe3O4 core-shell structural magnetic nanoparti-cles (FCSN) and analysis its characteristics. Methods :Fe3O4 nanoparticles were used to prepare FCSN by reduction-oxidation method. The characteristics of FCSN were determined by X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating specimen magnetometer (VSM). Using reverse-evaporation method to prepare 5-FU-FCSN magnetic targeted liposome and the best formula of magnetic liposome was obtained through the four factors of three levels orthogonal experiment The characteristics of magnetic liposome were measured with particle size analyzer(PCS) and TEM. Entrapment efficiency was determined by gel-chromatog-raphy. Results: FCSN was similar to a globe with the average particle size of 70 tun. XRD and TEM observation of FCSN shown that its shell was Fe3O4 and kernel was Fe, magnetic saturation intensity was 107.54 emu/g. 5-FU-FCSN magnetic liposome was similar to a globe with the average particle size of 202.5 nm, the entrapment efficiency of 33.5%. 5-FU-FCSN magnetic liposome was relatively stable in 4V, refrigerator after a month. Conclusion;5-FU-FCSN magnetic liposome was successfully prepared, which can be stored in 4t refrigerator for a long time.关键词
5-氟尿嘧啶/Fe/Fe3O4纳米颗粒/磁性脂质体Key words
5-fluorouracil/ Fe/Fe3O4 magnetic nanoparticles/ magnetic liposome分类
医药卫生引用本文复制引用
黄波涛,段磊,吴小玲,史慧慧,杨再兴,钟伟,戚晓红..5-氟尿嘧啶-Fe/Fe3O4磁性脂质体的制备及其表征[J].南京医科大学学报（自然科学版）,2011,31(7):951-955,1006,6.基金项目
国家863子课题合作项目(2007AA021805)；江苏省高校自然科学研究计划项目(08KJB310006) （2007AA021805）
