南京医科大学学报（自然科学版）2011，Vol.31Issue(6)：794-798,5.
紫杉醇对TGF-β1促人Tenon's囊成纤维细胞增殖的影响
Effect of paclitaxel on proliferation of human Tenon's capsule fibroblasts stimulated by TGF-β1
摘要
Abstract
Objective;To investigate the effect of TGF-β1 on the proliferation of human Tenon's capsule fibroblasts(HTFs) and the effect of Paclitaxel on the proliferation of HTFs stimulated by TGF-β1. Methods; ① HTFs cultured by tissue block adhering wall method were observed by optical microscope and identified by immunocytochemistry methods. ② The 4th and 6th generation of HTFs were incubated with various concentrations of TGF-β1 (0,2.5,5,10,25,50 ng/ml)for 24 hours. The proliferation of HTFs was evaluated by CCK-8. ③HTFs were treated with 10 ng/ml TGF-β1 and various concentration of Paclitaxel (0,10,100,1 000,10 000 nmol/L) for 24 h in order observe proliferation. ④ The cell apoptosis was measured by Hochest33342 staining. Results:① The Tenon's fibroblasts were successfully cultured. The results of vimentin immunocytochemisty staining was positive. ② Treatment of the cells with 2.5~25.0 ng/ml TGF-β1 increased the proliferation of the HTFs. ③ 100-10 000 nmol/L Paclitaxel suppressed the proliferation of the HTFs stimulated by TGF-β1. ④ Hochest staining shows a clear apoptotic features. Paclitaxel treatment resulted in increased number of apoptotic cells in a dose-dependent manner. Conclusion; TGF-β1 may increase the proliferation of HTFs, which could be reduced by Paclitaxel. Paclitaxel could bind to TGF-β1 receptor and interfere in signal transmission and inhibit the scarring after glaucoma filtering surgery.关键词
紫杉醇/转化生长因子-β1/青光眼/人Tenon's囊成纤维细胞Key words
Paclitaxel/TGF-β1/glaucoma/HTFs分类
生物科学引用本文复制引用
王荔,孙红,杨翎,袁志兰..紫杉醇对TGF-β1促人Tenon's囊成纤维细胞增殖的影响[J].南京医科大学学报（自然科学版）,2011,31(6):794-798,5.基金项目
江苏省教育厅自然科学基金(07KJB310070) （07KJB310070）
