肿瘤预防与治疗2011，Vol.24Issue(6)：281-283,3.DOI:10.3969/j.issn.1674-0904.2011.06.001
胃癌耐药细胞系BGC-823/CDDP耐药机制初步研究
The Study of Drug Resistance Mechanism of Cisplatin-resistant BGC-823/CDDP Cell Line
王攀 1魏寿江 1王崇树 1侯华芳1
作者信息
- 1. 川北医学院附属医院普外科,四川南充,637000
- 折叠
摘要
Abstract
O bjective: To establish a cisplatin- resistant hunan gastric cancer cell BGC-823/CDDP in vitrq and study its drug-resistantmechanisns M e1hods: By continuously exposing and gradually increasing the dose of cisplatin, a cisplatin-resistanthu-man gastric cancer cell line BGC-823/CDDP was established and it was frozened for3 months The drug sensitivity was measured by methythiazol tetrazoliin( MTT) assay, and P-Glycoprotein( P-gp) 、 p53、 B-cell Iymphona2( bc-2) ^ Topoisomerase ( TOP- Ⅱ ) and Glutathbne S epoxHe transferase( GST-π) protein were examined by mmunocytochemicalmethod Results; ByMTT assay, the resistance index of frozen BGC-823/CDDP cells toCDDP wasll. 17 and it also showed cross-resistance tomitonycinC ( MMQ , 5-flu-orouracil(5-Fu) and adriamycir( ADM). Compared to the parental BGC-823 cells, the expression ofGST-π protein was significantly up-regulated in BGC-823/CDDP( P <0. 01). The expressions of P-gp, p53-, bci-2 protein were negative in both wo cell lines ( P > 0.05). Conclisim: A stably resistant BGC-823/CDDP cell line with cross drug resistance is established in our experment; and GST-tt is related to cisplatin resistance of huamn gastric cancer关键词
胃癌细胞系/顺铂/药物耐受性Key words
Gastric Cancer Cell Line/ Cisplatin/ Drug Resistance分类
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王攀,魏寿江,王崇树,侯华芳..胃癌耐药细胞系BGC-823/CDDP耐药机制初步研究[J].肿瘤预防与治疗,2011,24(6):281-283,3.
