山西医科大学学报2011，Vol.42Issue(11)：861-863,937,4.DOI:10.3969/J.ISSN.1007-6611.2011.11.001
贝那普利对肝纤维化大鼠胰岛样生长因子结合蛋白-2表达的影响
Effect of benazepril on insulin-like growth factor binding protein-2 expression in hepatic fibrosis rats
赵彩霞 1申凤俊 1唐瑾1
作者信息
- 1. 山西医科大学第一临床医学院消化科,太原,030001
- 折叠
摘要
Abstract
Objective To explore the protective effects of angiotensin converting enzyme inhibitor( ACEI ) benazepril on insulin-like growth factor binding protein-2 ( IGFBP-2 ) expression in rats of hepatic fibrosis induced by carbon tetrachloride ( CC14). Methods Hepatic fibrosis was induced by intraperitoneal injection of carbon tetrachloride ( CC14 ) in rats. Twenty-two Wistar male rats were randomly divided into normal control group( n = 6 ), model group( n = 8 ), benazepril treatment group( n = 8 ). The rats in model group and benazepril treatment group were firstly given the intraperitoneal injection of 40% CC14 5 ml/kg,and followed by intraperitoneal injection of 40% CC14 2 ml/kg twice a week. The rats in control group were given intraperitoneal injection of the same dosage of oil. From the first day of intraperitoneal injection to the end of experiment, the rats in benazepril treatment group were given benazepril 10 mg/( kg·d) by gastric gavage. At the end of week 8, blood samples and liver tissues were collected. Histopathological changes of liver tissue were observed with hematoxylin-eosin ( HE ) and Masson trichrome staining. Serum IGFBP-2 levels were examined by enzyme-linked immu-nosorbent assay( ELISA ). IGFBP-2 expression in liver was evaluated by immunohistochemistry. Results The expression of IGFBP-2 in serum and liver tissues were significantly higher in model group than in normal control group( P ＜ 0. 05 ). Compared with model group, expression of IGFBP-2 in serum and liver tissues significantly decreased in benazepril treatment group ( P ＜0. 05 ). Benazepril attenuated the degree of hepatic fibrosis. Conclusion Benazepril can delay the degree of hepatic fibrosis by decreasing the expression IGFBP-2.关键词
肝纤维化/胰岛样生长因子结合蛋白-2/大鼠Key words
hepatic fibrosis/insulin-like growth factor binding protein-2/rats分类
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赵彩霞,申凤俊,唐瑾..贝那普利对肝纤维化大鼠胰岛样生长因子结合蛋白-2表达的影响[J].山西医科大学学报,2011,42(11):861-863,937,4.
