山西医科大学学报2011，Vol.42Issue(11)：931-934,942,5.DOI:10.3969/J.ISSN.1007-6611.2011.11.021
改良冠脉结扎法提高大鼠心梗模型存活率方法探讨
Modified method of ligating coronary for improving the survival rate of myocardial infarction rat model
王学文 1徐善慧 2张宁坤 2陈宇 2朱智明2
作者信息
- 1. 南方医科大学研究生学院,广州,510515
- 2. 北京海军总医院心脏中心
- 折叠
摘要
Abstract
Objective To explore a better way for improving the survival rate of myocardial infarction model rats. Methods The myocardial infarction model was produced by ligating left anterior descending branch coronary artery through left thoracotomy after injection of narcotic into abdominal cavity and trachea intubation via mouth. The rats were killed by injecting overdose of narcotic at week 4 after operation. The heart was taken out to observe the shape,infarct size and the pathological morphology by HE staining. Results The successful rate of modeling was 100% , and 96.7% of rats survived after operation. After operation for 4 weeks, the left ventricle became larger, the ischemic region became thinner and the endocardial tissues of the survival rats were fibrotic. Conclusion This modified method could improve the survival rate of animal model, and be used to make a stable model for the basic research of myocardial infarction, and reduce the bias in the experiment due to the unstable animal models.关键词
动物模型/心肌梗死/大鼠/呼吸机/麻醉Key words
animal model/myocardial infarction/rats/breathing machine/anesthesia分类
医药卫生引用本文复制引用
王学文,徐善慧,张宁坤,陈宇,朱智明..改良冠脉结扎法提高大鼠心梗模型存活率方法探讨[J].山西医科大学学报,2011,42(11):931-934,942,5.
