外科理论与实践2011，Vol.16Issue(4)：365-369,5.
正常人血浆成纤维细胞生长因子19与胆囊容积变化的关系
Study on the relationship between the plasma fibroblast growth factor 19 level and changes in gallbladder volume
摘要
Abstract
Objective Fibroblast growth factor 19 (FGF19) is involved physiologically in the refilling of gallbladder. To measure the expression of FCF19 in gallbladder tissue and to evaluate the relationship of the plasma FGF19 level and the changes of gallbladder volume. Methods mRNA level of FGF19 in gallbladder tissues were quantified by real-time PCR. Localization of FGF19 protein in gallbladder tissues was detected by immunohistochemistry. FGF19 in plasma was assayed by EliSA during fasting and 1-4 hour after fat-diet obtained from 4 female and 4 male healthy volunteers. At each time point, the gallbladder volume was measured using B-type ultrasonic scanning. Results mRNA of FGF19 was detected in gallbladder tissue. Immunohistochemistry showed that FGF19 protein was present in the epithelium of gallbladder. The gallbladder contracted after fat-diet and reached the maximum degree 2-hour after the diet in both sexes. In females, plasma FGF19 decreased 1-hour after the fat-diet, rose afterwards, and reached the peak 3-hour after the fat-diet. This change was earlier than the refilling of the gallbladder. However, similar findings were not observed in males. Conclusions Our study shows that FGF19 is highly expressed in the human gallbladder epithelium. Plasma FGF19 may be related with gallbladder refilling after fat-diet.关键词
成纤维细胞生长因子19；胆囊容积；收缩和舒张功能Key words
Fibroblast growth factor 19/Gallbladder volume/Contraction and refilling分类
医药卫生引用本文复制引用
雷铭,徐子平,蔡劬,计骏,王明亮,蒋兆彦,韩天权,张圣道..正常人血浆成纤维细胞生长因子19与胆囊容积变化的关系[J].外科理论与实践,2011,16(4):365-369,5.基金项目
国家自然科学基金(30700310，81070367) （30700310，81070367）
