陕西科技大学学报(自然科学版)2011,Vol.29Issue(3):37-40,4.
林麝熟制标本皮张DNA的提取及PCR分析
DNA EXTRACTION IN DRESSED SKIN OF MOSCHUS BEREZOVSKI FLEROV AND PCR ANALYSIS
摘要
Abstract
DNA extraction from animal specimens is significant for identification of animal species and investigation on variation and genetic diversity of animals. This paper exact DNA from dressed skin in Moschus berezovski flerov specimen made 50's last century. Cytochrome gene b was utilized in process of primer to amplify PCR. The result was sequenced and showed that specimen was of lower nuclear DNA content, better conserved mitochondrial DNA, and it could be amplified as DNA extracted from fresh flesh.关键词
林麝DNA;动物DNA;皮张DNA提取;PCR扩增;鲜肉DNAKey words
Moschus DNA/animal DNA/DNA of dressed skin/amplify PCR/DNA of fresh flesh分类
生物科学引用本文复制引用
冯慧,冯成利,刘晓农,任轶..林麝熟制标本皮张DNA的提取及PCR分析[J].陕西科技大学学报(自然科学版),2011,29(3):37-40,4.基金项目
陕西省科学院科技专项(2009K-02),陕西省重大科技专项(2010ZDKG-54) (2009K-02)