中国组织工程研究与临床康复2011，Vol.15Issue(40)：7416-7420,5.DOI:10.3969/j.issn.1673-8225.2011.40.002
血红素氧合酶1提高骨髓间充质干细胞在梗死后心脏的存活
Human heme oxygenase-1 improves the survival of bone marrow mesenchymal stem cells in ischemic heart
摘要
Abstract
BACKGROUND: Cardiac function is limited by the great amount of dead transplanted cells following myocardial infarction (AMI) treatment with bone marrow mesenchymal stem cells (BMSCs) transplantation.OBJECTIVE: To investigate the effects of human heme oxygenase-1 (HO-1) on the survival of BMSCs in the heart after AMI. METHODS: BMSCs were isolated, cultured and proliferated in vitro, transfected with Adv-hHO-1 and Adv-GFP, and then labeled with DAPI before transplantation. At 1 hour after left coronary artery ligation, DAPI-HO-1-BMSCs or DAPI-GFP-BMSCs were directly injected into the border of infracted cardiac region in rats. An equal volume of PBS was injected into the control group. RESULTS AND CONCLUSION: The expression of hHO-1 mRNA, vascular endothelial growth factor, basic fibroblast growth factor, hepatocyte growth factor in the border of infracted cardiac region treated with HO-1 -BMSCs were higher than those treated with GFP-BMSCs and PBS (P < 0.01), and the number of BMSCs and capillary vessels in AMI heart treated with HO-1-BMSCs was significantly higher than those treated with GFP-BMSCs and PBS (P < 0.05, P < 0.01). The heart function and remolding of the hearts treated with HO-1-BMSCs was better than those treated with GFP-BMSCs and PBS (P < 0.01). The infarct area in the HO-1-BMSCs group was also smaller than that in the GFP-BMSCs and PBS groups (P < 0.01). HO-1 enhances the survival of BMSCs in the AMI heart, which cooperates with BMSCs to inhibit ventricular remodeling and improve the cardiac function.关键词
血红素氧合酶1/骨髓间充质干细胞/心肌梗死/细胞因子/血管新生分类
基础医学引用本文复制引用
曾彬,易欣,马乐乐..血红素氧合酶1提高骨髓间充质干细胞在梗死后心脏的存活[J].中国组织工程研究与临床康复,2011,15(40):7416-7420,5.基金项目
国家自然科学基金资助项目(30900609). （30900609）
