中国组织工程研究与临床康复2011，Vol.15Issue(40)：7425-7428,4.DOI:10.3969/j.issn.1673-8225.2011.40.004
β-淀粉样蛋白诱导骨髓间充质干细胞向神经元样细胞分化过程中Tau蛋白的过度磷酸化
Beta-amyloid 25-35 causes Tau protein hyperphosphorylation of bone marrow mesenchymal stem cells differentiating into neuron-like cells
李俊 1赵天春 1段萍 1韩雪飞 1梁晨 1邢莹2
作者信息
- 1. 郑州大学基础医学院干细胞中心,河南省郑州市,450001
- 2. 新乡医学院,河南省新乡市,453003
- 折叠
摘要
Abstract
BACKGROUND: Many scholars believe that β-amyloid protein is the initial factor for the occurrence of Alzheimer's disease, andthe hyperphosphorylation of Tau protein may be one of the most important molecular pathological changes in Alzheimer'sdisease.OBJECTIVE: To study the effect of β-amyloid 25-35 (AG25-35) on Tau protein phosphorylation of rat bone marrowmesenchymal stem cells (BMSCs) differentiating into neuron-like cells.METHODS: BMSCs were isolated and purified from SD rats, and the fourth passage BMSCs were divided into two groups:AG25-35 experiment group was added pre-induction medium (containing 10 ug/L bFGF, 10% FBS in DMEM) and 20 u mol/LAB25-35, 24 hours later, the cells were induced into neurons-like cells by DMEM with 2% DMSO and 200 u mol/L BHA, andharvested 5 hours later; control group was treated with the same induction method but with no Aβ 25-35. Morphological changeswere observed by light microscope, neuron-specific enolase (NSE) detected by immunocytochemistry; GSK-3β,Tau[pSer262]and Tau [pSer396] are tested by Western blot.RESULTS AND CONCLUSION: With the observation of light microscope, spindle shaped BMSCs after induction showed upneuron-like cells shape with long projections, which were different from Aβ25-35 experiment group with the length and quantity ofthe projections shorter and less; both two groups showed Tau+ by immunocytochemistry; Western blotting showed that GSK-3β,Tau [pSer262] and Tau [pSer396] of Aβ 25-35 treatment group were significantly higher than those of the control group. Aβ25-35can induce BMSCs differentiating into neuron-like cells leading to the hyperphosphorylation of Tau protein through GSK-3.关键词
Tau蛋白/磷酸化/β-淀粉样蛋白/骨髓间充质干细胞/大鼠分类
基础医学引用本文复制引用
李俊,赵天春,段萍,韩雪飞,梁晨,邢莹..β-淀粉样蛋白诱导骨髓间充质干细胞向神经元样细胞分化过程中Tau蛋白的过度磷酸化[J].中国组织工程研究与临床康复,2011,15(40):7425-7428,4.
