首页|期刊导航|中国神经再生研究（英文版）|Stereological method for objectively quantifying myelin sheaths in the rat hippocampus
中国神经再生研究（英文版）2011，Vol.6Issue(31)：2405-2409,5.DOI:10.3969/j.issn.1673-5374.2011.31.001
Stereological method for objectively quantifying myelin sheaths in the rat hippocampus
Stereological method for objectively quantifying myelin sheaths in the rat hippocampus
摘要
Abstract
In the present study, tissue blocks were randomly sampled from the entire hippocampus of 6-week-old Long-Evans rats. Isotropic, uniform and random sections, 60 nm thick, were prepared by isector. Fifteen fields of view were randomly selected for each section and photographed using a transmission electron microscope. The mean internal and external diameters of the myelin sheaths were obtained by measuring the longest profile diameter perpendicular to its longest axis.The inner and outer perimeters of the myelin sheaths were estimated using the equidistant parallel test lines. The thickness of the myelin sheaths was estimated by direct orthogonal measurements in uniform, random locations. These stereological methods should permit an unbiased quantitative assessment of changes in the myelin sheaths of myelinated fibers in the hippocampus.关键词
myelin sheaths/diameter/perimeter/thickness/hippocampus/stereology/neural regenerationKey words
myelin sheaths/diameter/perimeter/thickness/hippocampus/stereology/neural regeneration引用本文复制引用
Lei Zhang, Wei Lu, Shu Yang,Lin Chen,Xuan Qiu,Guohua Cheng,Yong Tang..Stereological method for objectively quantifying myelin sheaths in the rat hippocampus[J].中国神经再生研究（英文版）,2011,6(31):2405-2409,5.基金项目
the National Natural Science Foundation of China,No.30973155 （）
