昆虫学报2011，Vol.54Issue(9)：975-981,7.
长镰管蚜气味结合蛋白OBP3的cDNA克隆和序列分析及其成虫嗅觉行为反应测定
cDNA cloning and sequence analysis of odorant binding protein OBP3 in Drepanosiphum platanoidis (Homoptera: Drepanosiphidae) and testing of its adult olfactory behavior
摘要
Abstract
ApisOBP3 from Acyrthosiphum pisum was known as its affinity to E-β-farnesene ( EBF) , and the volatile substances released by Drepanosiphum platanoidis contain no EBF which is a conventional component secreted by most of aphids. In this study, two odorant binding proteins named as DplaOBP3-l and DplaOBP3-2 sharing high identities (95. 8% and 98. 32%, respectively) with ApisOBP3 were identified in D. Platanoidis. The lengths of coding sequences of both DplaOBP3-1 and DplaOBP3-2 are 426 bp with 69 bp coding for signal peptide at the 5' end. DplaOBP3-l, DplaOBP3-2 and ApisOBP3 share 6 highly conserved cysteine residues at the same position among sequences. Based on the 3D structure of LmadPBP, a pheromone binding protein from cockroach Leucophaea maderae, we modeled the structures of DplaOBP3-l and DplaOBP3-2, and all the five hydrophobic amino acids and the aromatic amino acid residue, Tyr84, located in the binding cavity probably participating in interacting with ligands have their counterparts in the structure of ApisOBP3. Further, four-arm olfactometer assay showed that D. Platanoidis were significantly repelled by both EBF and crushed aphids (P =0. 01). The results suggest that volatiles from fresh crushed bodies could be the intraspecies alarm pheromone in D. Platanoidis; D. Platanoidis are repelled by allogenetic EBF whereas they never release EBF.关键词
枫长镰管蚜/气味结合蛋白/报警信息素/E-β-法尼烯(EBF)/cDNA克隆/四臂嗅觉仪Key words
Drepanosiphum platanoidis/odorant binding protein/alarm pheromone/E-β-farnesene (EBF)/cDNA cloning/four-arm olfactometer分类
生物科学引用本文复制引用
范佳,Sophie VANDERMOTEN,Frederic FRANCIS,刘勇,陈巨莲,程登发..长镰管蚜气味结合蛋白OBP3的cDNA克隆和序列分析及其成虫嗅觉行为反应测定[J].昆虫学报,2011,54(9):975-981,7.基金项目
国家自然科学基金项目(30971920) （30971920）
国家重大科技专项(2009ZX08012-007B) （2009ZX08012-007B）
国家小麦产业建设体系专项经费(nycytx-03) （nycytx-03）
中比合作项目(2010DFA32810,PICSHANDONG) （2010DFA32810,PICSHANDONG）
