中国组织工程研究与临床康复2011，Vol.15Issue(40)：7455-7458,4.DOI:10.3969/j.issn.1673-8225.2011.40.011
脐带结缔组织多潜能干细胞向内皮及心肌方向的分化
Differentiation of multipotent stem cells derived from Wharton's Jelly into endothelial cells and myocardial cells
谭强 1马春朋 1王庆胜 1齐羲明 1孙丽敏 1邹晓译 1柳东田 1卞秋武1
作者信息
- 1. 河北医科大学附属秦皇岛市第一医院心脏内科,河北省秦皇岛市066000
- 折叠
摘要
Abstract
BACKGROUND: There are plenty of multipotent stem cells in Wharton's Jelly that is a good stem cell resource. This kind of stemcell has the feasibility of low immunity and high proliferation.OBJECTIVE: To investigate the feasibility of multipotent stem cells derived from Wharton's Jelly differentiating into endothelialcells and myocardial cells.METHODS: Multipotent stem cells were isolated from Wharton's Jelly using the attached method.RESULTS AND CONCLUSION: Flow cytometer results showed that multipotent stem cells were positive for CD105, CD73,CD90, CD44, CD54, and negative for CD45, CD34, CD106 and CD133. Multipotent stem cells isolated from Wharton's Jellycould swallow ac-LDL and bind UEA-1 after addition of vascular endothelial growth factor and basic fibroblast factor. After5-azacytidine induction, troponin I and β-myoglobulin were positive in myocardial cells. The findings indicate that a mass ofstem cells can be obtained from Wharton's Jelly, which can differentiate into endothelial cells and myocardial ceWsin vitro.关键词
脐带/多潜能干细胞/结缔组织/内皮细胞/心肌细胞分类
医药卫生引用本文复制引用
谭强,马春朋,王庆胜,齐羲明,孙丽敏,邹晓译,柳东田,卞秋武..脐带结缔组织多潜能干细胞向内皮及心肌方向的分化[J].中国组织工程研究与临床康复,2011,15(40):7455-7458,4.
