中国组织工程研究与临床康复2011，Vol.15Issue(40)：7485-7488,4.DOI:10.3969/j.issn.1673-8225.2011.40.017
富血小板血浆促进脂肪间充质干细胞的体内外成骨
Promotional effects of autogeneic platelet-rich plasma on in vitro and in vivo osteoblastic ability of adipose-derived mesenchymal stem cells
摘要
Abstract
BACKGROUND: Platelet-rich plasma (PRP) can promote in vitro rapid transformation of osteoblastic phenotype.OBJECTIVE: To investigate the effects of PRP on in vitro and in vivo osteoblastic ability of adipose -derived mesenchymal stemcells (ADSCs).METHODS: ADSCs form rabbits in passage 3 were cultured by osteogenic induction culture medium (control group) orosteogenic induction culture medium containing 10 mL/L PRP. ADSCs of passage 3 were implanted intohydroxyapatite/tricalcium phosphate (HA/TCP) scaffold and cultured by osteogenic induction culture medium with or without10 mL/L PRP to detect the bone formation.RESULTS AND CONCLUSION: Alkaline phosphatase activity increased to the maximal level on day 14 in the PRP group and onday 18 in the control group, and then gradually declined, but remained a relative high level until to day 28. Histologically VonKossa staining showed that a lot of mineralized bone nodules which stained black adhered to the wall of inside porous scaffold intwo groups. A large number of calcium deposits were found. Toluidine blue dye showed that new bone formed in the pores, whichwas greater in the surrounding area than in the central area. The amount of in vitro calcium deposits was greater in the controlgroup, and the amount of new bone was more in the PRP group than the control group (P< 0.05). PRP could effectively speed upthe osteogenic differentiation of ADSCs in vitro and induce bone formation in vivo.关键词
富血小板血浆/脂肪间充质干细胞/成骨诱导/钙磷陶瓷支架/钙盐沉积分类
医药卫生引用本文复制引用
杨民,王剑,王强..富血小板血浆促进脂肪间充质干细胞的体内外成骨[J].中国组织工程研究与临床康复,2011,15(40):7485-7488,4.基金项目
国家自然科学基金项目(81171732) （81171732）
安徽省自然科学基金研究项目(070413091) （070413091）
安徽省医学科研课题(09C234) （09C234）
安徽省教育厅省级自然科学研究项目(KJ2007B041). （KJ2007B041）
