中国组织工程研究与临床康复2011，Vol.15Issue(40)：7497-7500,4.DOI:10.3969/j.issn.1673-8225.2011.40.020
白血病抑制因子抑制神经元凋亡及促进内源性神经干细胞的增殖
Leukemia inhibitory factor decreases the apoptosis of neurons and promotes the proliferation of endogenous neural stem cells
摘要
Abstract
BACKGROUND: Studies have shown that leukemia inhibitory factor (LIF) can enhance the proliferation of endogenous neural stem cells (ENSCs), and change their differentiation direction, but no studies have domenstrated whether LIF can affect neuronal apoptosis.OBJECTIVE: To detect the neuron apoptosis and the proliferation of ENSCs in mouse models after LIF administration. METHODS: Thirty-two c57BL mice were randomized into sham-operation group, LIF group, Parkinson's disease (PD) group and control group. Except the control group, micB in the other groups were injected with 6-hydroxydopamine to make PD models. LIF group received ALZET osmium pump duct implantation, and infused with normal saline containing LIF (25 ug/kg) into the cerebrospinal fluid. PD group received ALZET osmium pump duct implantation, and only infused with normal saline into the cerebrospinal fluid. Sham-operation group received ALZET osmium pump duct implantation without the pump duct. RESULTS AND CONCLUSION: LIF group showed a statistically significant improvement in motor functioin, compared with PD group; the number of ENSCs increased and the number of apoptosis neurons decreased significantly in the LIF group compared with PD group. It is possible that LIF is a neurotrophic factor for effectively rebuilding the degenerated central nervous system.关键词
帕金森病/内源性神经干细胞/白血病抑制因子/细胞凋亡/小鼠分类
基础医学引用本文复制引用
李亚丹,林玉凤,臧大维..白血病抑制因子抑制神经元凋亡及促进内源性神经干细胞的增殖[J].中国组织工程研究与临床康复,2011,15(40):7497-7500,4.基金项目
国家教育部回国人员科研启动基金资助(教外司留[2007]1108号),天津市科委课题资助(07JCYBJC09600). （教外司留[2007]1108号）
