中国组织工程研究与临床康复2011，Vol.15Issue(40)：7505-7510,6.DOI:10.3969/j.issn.1673-8225.2011.40.022
神经干细胞移植联用神经节苷脂对脑损伤大鼠神经学功能恢复的影响
Effects of ganglioside combined with neural stem cells transplantation on the neurologic function of rats with traumatic brain injury
摘要
Abstract
BACKGROUND: Ganglioside (GM1), through activation of neurotrophic factors, inhibits the damage of toxic products to neurons and reduces nerve cell death induced by excitatory amino acids to play a role in nerve cell repair.OBJECTIVE: To investigate the effect of neural stem cells (NSCs) transplantation and GM1 on traumatic brain injury (TBI). METHODS: Healthy Wistar rats were divided randomly into three groups: TBI group; NSCs transplantation group; NSCs transplantation+ GM1 group. AQP4 mRNA and AQP4 expressions were determined by RT-PCR and Western Blot at 4 day after TBI. The neurological defect scores were determined 24 hours, 3 days and 1, 2, 3, 4 weeks after TBI. The Morris water maze test was tested on days 21-28 after TBI. The immunohistochemistry and pathology changes were checked after 4 weeks. RESULTS AND CONCLUSION: The expressions of AQP4 mRNA and AQP4 were highest in the TBI group, higher in the NSCs transplantation group and lowest in the NSCs transplantation+ GM1 group (P < 0.05). At 1, 2, 3 and 4 weeks after TBI, the neurological defect scores were lower in the NSCs transplantation group than those in the TBI group (P< 0.05), and significantly lower in the NSCs transplantation+GM1 group than those in the TBI group (P < 0.01); The average time of escape latency was gradually decreased in each group. However, from the 3 to 5 day, it was much shorter in the NSCs transplantation+GM1 group than in the TBI group (P < 0.01) and shorter in the NSCs transplantation+GM1 group than in NSCs transplantation group (P< 0.05). In addition, the frequency platform passing in the NSCs transplantation+GM1 group and the percentage of swimming distance traveled in the previous target quadrant was significantly greater than those of the TBI group and NSCs transplantation group. The number of neurons in the NSCs transplantation+GM1 group was higher than that in the NSCs transplantation group and TBI group (P < 0.05). GM1 combined with neural stem cells transplantation can significantly improve the neurological function in the rats with TBI.关键词
脑损伤/神经干细胞/移植/神经节苷脂/大鼠分类
医药卫生引用本文复制引用
李红星,苑国富,金耀东,王静,赵宗茂..神经干细胞移植联用神经节苷脂对脑损伤大鼠神经学功能恢复的影响[J].中国组织工程研究与临床康复,2011,15(40):7505-7510,6.基金项目
河北省医学科学研究重点课题计划(20110598). （20110598）
