中国组织工程研究与临床康复2011，Vol.15Issue(40)：7549-7553,5.DOI:10.3969/j.issn.1673-8225.2011.40.032
三维培养条件下胚胎鼠唾液腺上皮细胞的自组装
Self-organization and branching morphogenesis of primary salivary epithelial cells under three-dimensional culture
摘要
Abstract
BACKGROUND: To understand self-assembly and branching of primary salivary epithelial cells will provide insights intoreconstructing artifical salivary gland orgnoid.OBJECTIVE: To investigate the machanism of self-organization and branching mophogenesis of primary submandibularepithelial cells under three-dimensional condition.METHODS: The embryonic day 15 (E 15) mouse submandibular gland epithelium were inoculated in matrigel for 3-dimantionalculture with/without (Group A/Group B) anti-E-cadherin and and anti-β-catenin. The contructs of these cells aggregation wereobserved under inverted microscope at days 1,6, 12. The contructs were observed with cytokeratin 19 immunohistochemistrystaining, and identified with Cadherin and β-catenin immunostaining and confocal microscopy.MAIN OUTCOME MEASURES: The constructs were observed under inverted microscope and analyzed by antibody ofcytokeratin 19 with immunohistochemistry, and identified with cadherin and G-catenin immunostaining and confocal microscopy.In Group A, branching morphogenesis and acinar-like structure were not observed. In Group B, these contructs could be formedat day 12. In Group A, weak green fruorescence of E-cadherin and β-catenin expressed weakly. In Group B, they expressedstrongly, and in the cytoplasm. MOD was lower in Group A than Group B (P < 0.01). E-cadherin and β-catenin are two importantfactors during the embryonic submandibular salivary gland epithelium self-organization关键词
下颌下腺上皮细胞/胚胎鼠/三维培养/自组装/E-cadherin/β-catenin分类
基础医学引用本文复制引用
王军胜,黄桂林,张霓霓,姚礼,唐岳鹏..三维培养条件下胚胎鼠唾液腺上皮细胞的自组装[J].中国组织工程研究与临床康复,2011,15(40):7549-7553,5.基金项目
贵州省优秀科技教育人才省长资金[黔省专合字(2008)113号] （2008）
贵州省教育厅基金[(2006)354号]. （2006）
