成都中医药大学学报Issue(3)：32-35,4.
早期实验性近视眼视网膜色素上皮细胞的培养及超微结构变化的研究
Observation of Ultrastructure and Culture of RPE of Early Experimental Myopia
摘要
Abstract
Objective: To analyze the biological characteristics of guinea pig anterior and posterior retinal pigment epithelial cells. Methods: 30 two - week - old guinea pigs which were divided in three groups randomly were lens - induced monocularly, A spherical lenses of -10. 00D were wear to unilateral eyes, another eye in each animal was untreated as self - control ( SC) .5 two -week - old guinea pigs were randomly closed as normal - control ( NC) . Each group was raised for 6 、 15 , 30 days separately, Before and after experiment, refraction was measured using retinoscopy and the oculus uterque axis oculi length determined by A - scan ultra-sonography. The anterior and posterior retinal pigment epithelial cells of each group were cultured and passaged 3-6 generations in vitro. The cultured cells were studiedby light microscope and electron microscope. Results: The endoplasmic reticulum dilation and Organelles such as endoplasmic reticulum and Ribosome decrease of anterior and posterior retinal pigment epithelial cells were observed after IS and 6 days induced. Conclusins: Cell proliferation of the anterior retinal pigment epithelial cells is reduced after 15 days induced , the same result happens in the posterior retinal pigment epithelial cells after 6 days induced.关键词
豚鼠；视网膜色素上皮细胞/超微结构Key words
Guinea pig/ retinal pigment epithelial cells/ experimental myopia分类
医药卫生引用本文复制引用
陈博宇,王超英,安慧琴,马景学,陈维毅,郝岚,刘迎庆..早期实验性近视眼视网膜色素上皮细胞的培养及超微结构变化的研究[J].成都中医药大学学报,2011,(3):32-35,4.基金项目
国家自然科学基金(批准号：10872140) （批准号：10872140）
