中国人兽共患病学报2011，Vol.27Issue(9)：774-777,786,5.
肺孢子虫(菌)p55抗原嵌合基因的克隆及表达产物分析
Cloning of chimeric gene of p55 antigen from Pneumocystis and analysis on the expression Product
摘要
Abstract
To construct the prokaryotic expression vector of chimeric gene of p55 antigen from Pneumocystis and to analyze and identify the expression product, the possible epitopes of p55 protein and its variants were forecasted by the bioinforma-tics technology and a polypeptide containing multiple possible epitopes was designed. The amino acid sequences of the polypep-tide were converted to the nucleotide sequences according to the genetic center ruler and the codon preference of E. Coli to make the codon optimization. The chimeric gene(CAG) fragment was synthesized and inserted into the plasmid Pgex-6p-l (containing glutathione S- transferase; GST) to construct the recombinant plasmid. The expression of the recombinant protein in E. Coli was induced by IPTG. The recombinant protein was analysized and identified by SDS-polyacrylamide gel electrophoresis and western-blotting. The recombinant plasmid Pgex-6p-l/CAG was constructed successfully. The relative molecular weight of the recombinant protein was about 69 000. The result of Western-blotting showed the recombinant protein was recognized by anti-GST. Tfs suggested that the prokaryotic expression vector Pgex-6p-l/CAG was constructed successfully and the prokaryotic expression system was established.关键词
肺孢子菌/p55蛋白/嵌合基因/重组融合蛋白Key words
Pneumocystis/ p55 protein/ chimeric gene/ recombinant fusion protein分类
医药卫生引用本文复制引用
王雪莲,栾和芝,刘彤,赵雨杰,安春丽..肺孢子虫(菌)p55抗原嵌合基因的克隆及表达产物分析[J].中国人兽共患病学报,2011,27(9):774-777,786,5.基金项目
国家自然基金资助项目(No.30670916) （No.30670916）
留学回国人员科研启动基金资助项目(No.[2010] 1174) （No.[2010] 1174）
辽宁省教育厅项目(No.L2010579) （No.L2010579）
